Anti-pro Caspase-9 antibody [JJ08-05]
cat.: ET1701-22
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB, IHC-P, FC
Clonality: Monoclonal
Clone number: JJ08-05
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Protein A purified.
Molecular weight: 46 kDa
Isotype: IgG
Immunogen: recombinant protein
Positive control: Hela, human liver cancer tissue.
Subcellular location: Cytosol,Nucleus.
Recommended Dilutions:
  WB
  FC
  IHC-P

1:1,000-1:2,000
1:10-1:50
1:50-1:200
Uniprot #: P55211(Human)
Alternative names: APAF-3 antibody APAF3 antibody Apoptotic protease Mch-6 antibody Apoptotic protease-activating factor 3 antibody CASP-9 antibody CASP9 antibody CASP9_HUMAN antibody Caspase-9 subunit p10 antibody Caspase-9 antibody Caspase 9 antibody Caspase9 antibody ICE-LAP6 antibody ICE-like apoptotic protease 6 antibody ICELAP6 antibody MCH6 antibody
Images
ET1701-22_1.jpg Fig1: Western blot analysis of pro Caspase 9 on Hela cells lysates using anti-pro Caspase 9 antibody at 1/1,000 dilution.
ET1701-22_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-pro Caspase-9 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1701-22, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1701-22_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human skin tissue using anti-pro Caspase-9 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1701-22, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1701-22_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human cervix uteri tissue using anti-pro Caspase-9 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1701-22, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1701-22_5.jpg Fig5: Flow cytometric analysis of Jurkat cells with pro Caspase 9 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.