pro Caspase-9 Recombinant Rabbit Monoclonal Antibody [JJ08-05]
cat.: ET1701-22
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB, IHC-P, FC
Clonality: Monoclonal
Clone number: JJ08-05
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 46 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human Caspase-9 aa 20-69 / 416.
Positive control: Hela cells lysates, human placenta tissue, human skin tissue, human cervix uteri tissue, Jurkat.
Subcellular location: Cytosol,Nucleus.
Recommended Dilutions:
  WB
  IHC-P
  FC

1:1,000-1:2,000
1:50-1:200
1:10-1:50
Uniprot #: SwissProt: P55211 Human
Alternative names: Caspase9 APAF-3 APAF3 Apoptotic protease Mch-6 Apoptotic protease-activating factor 3 CASP-9 CASP9 CASP9_HUMAN Caspase-9 subunit p10 Caspase-9 Caspase 9 Caspase9 ICE-LAP6 ICE-like apoptotic protease 6 ICELAP6 MCH6
Images
ET1701-22_1.jpg Fig1: Western blot analysis of pro Caspase 9 on Hela cells lysates using anti-pro Caspase 9 antibody at 1/1,000 dilution.
ET1701-22_2.jpg Fig2: Western blot analysis of pro Caspase-9 on different lysates with Rabbit anti-pro Caspase-9 antibody (ET1701-22) at 1/1,000 dilution.

Lane 1: Hela-si NT cell lysate
Lane 2: Hela-si Caspase-9 cell lysate

Lysates/proteins at 10 µg/Lane.

Predicted band size: 46 kDa
Observed band size: 46 kDa

Exposure time: 5 minutes;

4-20% SDS-PAGE gel.

ET1701-22 was shown to specifically react with Caspase-9 in Hela-si NT cells. Weakened band was observed when Hela-si Caspase-9 sample was tested. Hela-si NT and Hela-si Caspase-9 samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ET1701-22, 1/1,000) and Loading control antibody (Rabbit anti-GAPDH, ET1601-4, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-rabbit IgG-HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
ET1701-22_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-pro Caspase-9 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1701-22, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1701-22_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human skin tissue using anti-pro Caspase-9 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1701-22, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1701-22_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human cervix uteri tissue using anti-pro Caspase-9 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1701-22, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1701-22_6.jpg Fig6: Flow cytometric analysis of Jurkat cells with pro Caspase 9 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.