ET1701-24

CD36 Recombinant Rabbit Monoclonal Antibody [JJ2005]

cat.: ET1701-24

Product Type:	Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity:	Human, Mouse, Rat
Applications:	WB, IF-Cell, IF-Tissue, IHC-P, FC, IP
Clonality:	Monoclonal
Clone number:	JJ2005

Form:	Liquid
Storage condition:	Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer:	1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration:	1ug/ul
Purification:	Protein A affinity purified.
Molecular weight:	Predicted band size: 53 kDa
Isotype:	IgG
Immunogen:	Synthetic peptide within Human CD36 aa 360-373 / 472.
Positive control:	Human heart tissue lysate, mouse heart tissue lysate, RAW264.7, human spleen tissue, mouse spleen tissue, mouse heart tissue, mouse lung tissue, mouse stomach tissue, THP-1.
Subcellular location:	Cell membrane, Membrane raft, Golgi apparatus, Apical cell membrane.
Recommended Dilutions: WB IF-Cell IF-Tissue IHC-P FC IP	1:500-1:1,000 1:50-1:200 1:50-1:200 1:50-1:200 1:50-1:100 Use at an assay dependent concentration.
Uniprot #:	SwissProt: P16671 Human \| Q08857 Mouse \| Q07969 Rat
Alternative names:	Adipocyte membrane protein BDPLT10 CD36 CD36 antigen (collagen type I receptor, thrombospondin receptor) CD36 antigen CD36 molecule (thrombospondin receptor) CD36 molecule CD36_HUMAN CHDS7 Cluster determinant 36 Collagen receptor, platelet FAT Fatty acid translocase Fatty acid transport protein Glycoprotein IIIb GP IIIb GP3B GP4 GPIIIB GPIV Leukocyte differentiation antigen CD36 MGC108510 MGC91634 PAS 4 protein PAS IV PAS-4 PASIV Platelet collagen receptor Platelet glycoprotein 4 Platelet glycoprotein IV scarb3 Scavenger receptor class B member 3 Thrombospondin receptor

Images

	Fig1: Western blot analysis of CD36 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1701-24, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Human heart tissue lysate Lane 2: Mouse heart tissue lysate
	Fig2: Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-CD36 antibody (ET1701-24) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ET1701-24) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig3: Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Rabbit anti-CD36 antibody (ET1701-24) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ET1701-24) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

	Fig4: Immunohistochemical analysis of paraffin-embedded mouse heart tissue with Rabbit anti-CD36 antibody (ET1701-24) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ET1701-24) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig5: Immunohistochemical analysis of paraffin-embedded mouse lung tissue with Rabbit anti-CD36 antibody (ET1701-24) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ET1701-24) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig6: Immunohistochemical analysis of paraffin-embedded mouse stomach tissue with Rabbit anti-CD36 antibody (ET1701-24) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ET1701-24) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Fig7: Flow cytometric analysis of CD36 was done on THP-1 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1701-24, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Fig8: Immunocytochemistry analysis of THP-1 cells treated with 100ng/mL PMA for 72 hours labeling CD36 with Rabbit anti-CD36 antibody (ET1701-24) at 1/100 dilution.

Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CD36 antibody (ET1701-24) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.