Cdk7 Recombinant Rabbit Monoclonal Antibody [JJ203-01]
cat.: ET1701-25
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human
Applications: IF-Cell, FC
Clonality: Monoclonal
Clone number: JJ203-01
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 39 kDa
Isotype: IgG
Immunogen: Recombinant protein within Human Cdk7 aa 151-346 / 346.
Positive control: HeLa, A431, HepG2, 293.
Subcellular location: Nucleus, Cytoplasm, perinuclear region.
Recommended Dilutions:
  IF-Cell
  FC
1:100-1:500
1:50-1:100
Uniprot #: SwissProt: P50613 Human
Alternative names: 39 kDa protein kinase CAK CAK1 Cdk activating kinase CDK-activating kinase 1 CDK-activating kinase cdk7 CDK7_HUMAN CDKN7 Cell division protein kinase 7 Cyclin dependent kinase 7 cyclin-dependent kinase 7 (MO15 homolog, Xenopus laevis, cdk-activating kinase) Cyclin-dependent kinase 7 HCAK Homolog of Xenopus MO15 Cdk activating kinase Kinase subunit of CAK MO15 MO15, Xenopus, homolog of P39 Mo15 p39MO15 Serine threonine kinase Stk1 Serine/threonine protein kinase 1 Serine/threonine protein kinase MO15 STK1 TFIIH basal transcription factor complex kinase subunit
Images
ET1701-25_1.jpg Fig1: Immunocytochemistry analysis of HeLa cells labeling Cdk7 with Rabbit anti-Cdk7 antibody (ET1701-25) at 1/100 dilution.

Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Cdk7 antibody (ET1701-25) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
ET1701-25_2.jpg Fig2: ICC staining of Cdk7 in A431 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1701-25, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1701-25_3.jpg Fig3: ICC staining of Cdk7 in HepG2 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1701-25, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1701-25_4.jpg Fig4: ICC staining of Cdk7 in 293 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1701-25, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1701-25_5.jpg Fig5: Flow cytometric analysis of Cdk7 was done on A431 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1701-25, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.