AGO3 Recombinant Rabbit Monoclonal Antibody [JJ201-07]
cat.: ET1701-26
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IF-Tissue, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | JJ201-07 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 97 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human EIF2C3/ Argonaute 3 aa 689-860/860. |
| Positive control: | HeLa cell lysate, C6 cell lysate, N2A, SHG-44, F9, NCCIT, rat spinal cord tissue, rat brain tissue, mouse brain tissue, rat testis tissue. |
| Subcellular location: | Cytoplasm,Nucleus. |
| Recommended Dilutions:
WB IF-Cell IF-Tissue IHC-P FC |
1:1,000 1:100-1:500 1:100-1:500 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: Q9H9G7 Human | Q8CJF9 Mouse Unigene: 92025 Rat |
| Alternative names: | 5730550L01Rik Ago 3 Ago3 AGO3_HUMAN argonaute 3 Argonaute3 eIF 2C 3 eIF-2C 3 eIF2C 3 Eif2c3 EIF2C3 protein Eukaryotic translation initiation factor 2C 3 Eukaryotic translation initiation factor 2C3 FLJ12765 hAgo3 MGC86946 Protein argonaute-3 |
Images
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Fig1:
Western blot analysis of AGO3 on different lysates with Rabbit anti-AGO3 antibody (ET1701-26) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: C6 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 97 kDa Observed band size: 97 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1701-26) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2: ICC staining AGO3 in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining AGO3 in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: ICC staining AGO3 in F9 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig5: ICC staining AGO3 in NCCIT cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig6: Immunohistochemical analysis of paraffin-embedded rat spinal cord tissue using anti-AGO3 antibody. Counter stained with hematoxylin. |
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Fig7: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-AGO3 antibody. Counter stained with hematoxylin. |
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Fig8: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-AGO3 antibody. Counter stained with hematoxylin. |
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Fig9: Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-AGO3 antibody. Counter stained with hematoxylin. |
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Fig10: Flow cytometric analysis of N2A cells with AGO3 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.