Phospho-GluR1 (S845) Recombinant Rabbit Monoclonal Antibody [JJ2009]
cat.: ET1701-28
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse, Rat, Human |
| Applications: | WB, FC |
| Clonality: | Monoclonal |
| Clone number: | JJ2009 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 102 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic phospho-peptide corresponding to residues surrounding Ser845 of Human GluR1 aa 831-880 / 906. |
| Positive control: | Mouse brain tissue lysate, rat brain tissue lysate, mouse brain tissue lysate the membrane treated with λpp for 1 hour, rat brain tissue lysate the membrane treated with λpp for 1 hour, N2A. |
| Subcellular location: | Cell membrane, Endoplasmic reticulum membrane, Cell junction, Cell projection. |
| Recommended Dilutions:
WB FC |
1:1,000 1:10-1:50 |
| Uniprot #: | SwissProt: P42261 Human | P23818 Mouse | P19490 Rat |
| Alternative names: | GLUR 1 GLUR A AMPA 1 AMPA selective glutamate receptor 1 AMPA-selective glutamate receptor 1 GluA1 GLUH1 GluR K1 GluR-1 GluR-A GluR-K1 GLUR1 GLURA GluRK1 Glutamate receptor 1 Glutamate receptor ionotropic AMPA 1 Glutamate receptor ionotropic Glutamate receptor, ionotropic, AMPA 1 Gria1 GRIA1_HUMAN HBGR1 MGC133252 OTTHUMP00000160643 OTTHUMP00000165781 OTTHUMP00000224241 OTTHUMP00000224242 OTTHUMP00000224243 |
Images
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Fig1:
Western blot analysis of Phospho-GluR1 (S845) on different lysates with Rabbit anti-Phospho-GluR1 (S845) antibody (ET1701-28) at 1/1,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Rat brain tissue lysate Lane 3: Mouse brain tissue lysate, the membrane treated with λpp for 1 hour Lane 4: Rat brain tissue lysate, the membrane treated with λpp for 1 hour Lysates/proteins at 40 µg/Lane. Predicted band size: 102 kDa Observed band size: 102 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1701-28) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2: Flow cytometric analysis of Phospho-GluR1 (S845) was done on N2A cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1701-28, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.