Phospho-GluR1 (S845) Recombinant Rabbit Monoclonal Antibody [JJ2009]
cat.: ET1701-28
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Mouse, Rat, Human
Applications: WB, FC
Clonality: Monoclonal
Clone number: JJ2009
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 102 kDa
Isotype: IgG
Immunogen: Synthetic phospho-peptide corresponding to residues surrounding Ser845 of Human GluR1 aa 831-880 / 906.
Positive control: Mouse brain tissue lysate, rat brain tissue lysate, mouse brain tissue lysate the membrane treated with λpp for 1 hour, rat brain tissue lysate the membrane treated with λpp for 1 hour, N2A.
Subcellular location: Cell membrane, Endoplasmic reticulum membrane, Cell junction, Cell projection.
Recommended Dilutions:
  WB
  FC

1:1,000
1:10-1:50
Uniprot #: SwissProt: P42261 Human | P23818 Mouse | P19490 Rat
Alternative names: GLUR 1 GLUR A AMPA 1 AMPA selective glutamate receptor 1 AMPA-selective glutamate receptor 1 GluA1 GLUH1 GluR K1 GluR-1 GluR-A GluR-K1 GLUR1 GLURA GluRK1 Glutamate receptor 1 Glutamate receptor ionotropic AMPA 1 Glutamate receptor ionotropic Glutamate receptor, ionotropic, AMPA 1 Gria1 GRIA1_HUMAN HBGR1 MGC133252 OTTHUMP00000160643 OTTHUMP00000165781 OTTHUMP00000224241 OTTHUMP00000224242 OTTHUMP00000224243
Images
ET1701-28_1.jpg Fig1: Western blot analysis of Phospho-GluR1 (S845) on different lysates with Rabbit anti-Phospho-GluR1 (S845) antibody (ET1701-28) at 1/1,000 dilution.

Lane 1: Mouse brain tissue lysate
Lane 2: Rat brain tissue lysate
Lane 3: Mouse brain tissue lysate, the membrane treated with λpp for 1 hour
Lane 4: Rat brain tissue lysate, the membrane treated with λpp for 1 hour

Lysates/proteins at 40 µg/Lane.

Predicted band size: 102 kDa
Observed band size: 102 kDa

Exposure time: 3 minutes; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1701-28) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
ET1701-28_2.jpg Fig2: Flow cytometric analysis of Phospho-GluR1 (S845) was done on N2A cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1701-28, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.