Phospho-Raf1 (S621) Recombinant Rabbit Monoclonal Antibody [JJ085-05]
cat.: ET1701-3
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Rat, Mouse |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JJ085-05 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 73 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic phospho-peptide corresponding to residues surrounding Ser621 of Human Raf1 aa 591-640 / 648. |
| Positive control: | Human skeletal muscle tissue lysates, rat trachea tissue, human muscle tissue, human kidney tissue. |
| Subcellular location: | Cytoplasm, Cell membrane, Mitochondrion, Nucleus. |
| Recommended Dilutions:
WB IHC-P |
1:1,000-1:2,000 1:100-1:500 |
| Uniprot #: | SwissProt: P04049 Human | Q99N57 Mouse | P11345 Rat |
| Alternative names: | c Raf C-raf C-Raf proto-oncogene, serine/threonine kinase CMD1NN Craf 1 transforming gene cRaf Craf1 transforming gene EC 2.7.11.1 kinase Raf1 Murine sarcoma 3611 oncogene 1 NS5 Oncogene MIL Oncogene RAF1 OTTHUMP00000160218 OTTHUMP00000207813 OTTHUMP00000209389 Protein kinase raf 1 Proto-oncogene c-RAF Raf 1 Raf 1 proto oncogene serine/threonine kinase RAF Raf proto oncogene serine/threonine protein kinase RAF proto-oncogene serine/threonine-protein kinase RAF-1 RAF1 RAF1_HUMAN Similar to murine leukemia viral (V-raf-1) oncogene homolog 1 TRANSFORMING REPLICATION-DEFECTIVE MURINE RETROVIRUS 3611-MSV v raf 1 murine leukemia viral oncogene homolog 1 v-raf murine sarcoma viral oncogene homolog 1 v-raf-1 murine leukemia viral oncogene-like protein 1 vraf1 murine leukemia viral oncogene homolog 1 |
Images
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Fig1: Western blot analysis of Phospho-Raf1 (S621) on human skeletal muscle tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1701-3, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. |
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Fig2: Immunohistochemical analysis of paraffin-embedded rat trachea tissue using anti-Phospho-Raf1 (S621) antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1701-3, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3: Immunohistochemical analysis of paraffin-embedded human muscle tissue using anti-Phospho-Raf1 (S621) antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1701-3, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Phospho-Raf1 (S621) antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1701-3, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.