Anti-ATF7 antibody [JJ2-072]
cat.: ET1701-31
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, ICC/IF, IHC-P, FC
Clonality: Monoclonal
Clone number: JJ2-072
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Protein A affinity purified.
Molecular weight: 53 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human ATF7 aa 1-50 / 483.
Positive control: Hela, HepG2, mouse lung tissue, mouse skeletal muscle tissue, mouse heart tissue.
Subcellular location: Nucleus, Cytoplasm.
Recommended Dilutions:
  WB
  ICC/IF
  IHC-P
  FC

1:500-1:1,000
1:50-1:200
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: P17544 Human | Q8R0S1 Mouse
Unigene: 219706 Rat
Alternative names: 1110012F10Rik 9430065F09Rik Activating transcription factor 7 AI549878 ATF7 Atf7 protein ATF7_HUMAN ATFA C130020M04Rik cAMP-dependent transcription factor ATF-7 Cyclic AMP-dependent transcription factor ATF 7 Cyclic AMP-dependent transcription factor ATF-7 MGC31554 MGC57182 Transcription factor ATF A Transcription factor ATF-A
Images
ET1701-31_1.jpg Fig1: ICC staining ATF7 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ET1701-31_2.jpg Fig2: ICC staining ATF7 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ET1701-31_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded mouse lung tissue using anti-ATF7 antibody. Counter stained with hematoxylin.
ET1701-31_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue using anti-ATF7 antibody. Counter stained with hematoxylin.
ET1701-31_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-ATF7 antibody. Counter stained with hematoxylin.
ET1701-31_6.jpg Fig6: Flow cytometric analysis of Jurkat cells with ATF7 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.