ATF7 Recombinant Rabbit Monoclonal Antibody [JJ2-072]
cat.: ET1701-31
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IF-Tissue, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | JJ2-072 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 52 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human ATF7 aa 1-50 / 483. |
| Positive control: | HeLa cell lysate, Raji cell lysate, RAW264.7 cell lysate, PC-12 cell lysate, HepG2, Hela, mouse lung tissue, mouse skeletal muscle tissue, mouse heart tissue. |
| Subcellular location: | Nucleus, Cytoplasm. |
| Recommended Dilutions:
WB IF-Cell IF-Tissue IHC-P FC |
1:1,000 1:50-1:200 1:50-1:200 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: P17544 Human | Q8R0S1 Mouse Unigene: 219706 Rat |
| Alternative names: | 1110012F10Rik 9430065F09Rik Activating transcription factor 7 AI549878 ATF7 Atf7 protein ATF7_HUMAN ATFA C130020M04Rik cAMP-dependent transcription factor ATF-7 Cyclic AMP-dependent transcription factor ATF 7 Cyclic AMP-dependent transcription factor ATF-7 MGC31554 MGC57182 Transcription factor ATF A Transcription factor ATF-A |
Images
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Fig1:
Western blot analysis of ATF7 on different lysates with Rabbit anti-ATF7 antibody (ET1701-31) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: Raji cell lysate Lane 3: RAW264.7 cell lysate Lane 4: PC-12 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 52 kDa Observed band size: 55 kDa Exposure time: 1 minute 59 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1701-31) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2: ICC staining ATF7 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining ATF7 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: Immunohistochemical analysis of paraffin-embedded mouse lung tissue using anti-ATF7 antibody. Counter stained with hematoxylin. |
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Fig5: Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue using anti-ATF7 antibody. Counter stained with hematoxylin. |
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Fig6: Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-ATF7 antibody. Counter stained with hematoxylin. |
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Fig7: Flow cytometric analysis of Jurkat cells with ATF7 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.