Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
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Species reactivity: | Human, Mouse, Rat |
Applications: | WB, IF-Cell, IF-Tissue, IHC-P, FC |
Clonality: | Monoclonal |
Clone number: | JJ2-072 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Protein A affinity purified. |
Molecular weight: | Predicted band size: 52 kDa |
Isotype: | IgG |
Immunogen: | Synthetic peptide within Human ATF7 aa 1-50 / 483. |
Positive control: | Hela, HepG2, mouse lung tissue, mouse skeletal muscle tissue, mouse heart tissue. |
Subcellular location: | Nucleus, Cytoplasm. |
Recommended Dilutions:
WB IF-Cell IF-Tissue IHC-P FC |
1:500-1:1,000 1:50-1:200 1:50-1:200 1:50-1:200 1:50-1:100 |
Uniprot #: | SwissProt: P17544 Human | Q8R0S1 Mouse Unigene: 219706 Rat |
Alternative names: | 1110012F10Rik 9430065F09Rik Activating transcription factor 7 AI549878 ATF7 Atf7 protein ATF7_HUMAN ATFA C130020M04Rik cAMP-dependent transcription factor ATF-7 Cyclic AMP-dependent transcription factor ATF 7 Cyclic AMP-dependent transcription factor ATF-7 MGC31554 MGC57182 Transcription factor ATF A Transcription factor ATF-A |
Fig1: ICC staining ATF7 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. | |
Fig2: ICC staining ATF7 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. | |
Fig3: Immunohistochemical analysis of paraffin-embedded mouse lung tissue using anti-ATF7 antibody. Counter stained with hematoxylin. | |
Fig4: Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue using anti-ATF7 antibody. Counter stained with hematoxylin. |
Fig5: Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-ATF7 antibody. Counter stained with hematoxylin. | |
Fig6: Flow cytometric analysis of Jurkat cells with ATF7 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody |