Phospho-PLK1 (T210) Recombinant Rabbit Monoclonal Antibody [JJ20-58]
cat.: ET1701-33
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IF-Cell, IF-Tissue, FC, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JJ20-58 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 68 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic phospho-peptide corresponding to residues surrounding Thr210 of Human PLK1 aa 181-230 / 603. |
| Positive control: | HeLa treated with 100nM Nocodazole for 24 hours cell lysate, HeLa treated with 2mM thymidine for 16 hours then treated with 100nM Nocodazole for 24 hours cell lysate, HT-29 treated with 100nM Nocodazole for 24 hours cell lysate, HT-29 treated with 2mM thymidine for 16 hours then treated with 100nM Nocodazole for 24 hours cell lysate, mouse thymus tissue, MCF-7. |
| Subcellular location: | Nucleus, Chromosome, Cytoplasm, Midbody. |
| Recommended Dilutions:
WB IF-Cell IF-Tissue FC IHC-P |
1:1,000 1:10-1:50 1:10-1:50 1:10-1:50 1:200 |
| Uniprot #: | SwissProt: P53350 Human | Q07832 Mouse |
| Alternative names: | Cell cycle regulated protein kinase PLK 1 PLK PLK-1 plk1 PLK1_HUMAN Polo like kinase 1 Polo-like kinase 1 Serine/threonine protein kinase 13 Serine/threonine protein kinase PLK1 Serine/threonine-protein kinase 13 Serine/threonine-protein kinase PLK1 STPK 13 STPK13 |
Images
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Fig1:
Western blot analysis of Phospho-PLK1 (T210) on different lysates with Rabbit anti-Phospho-PLK1 (T210) antibody (ET1701-33) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 100nM Nocodazole for 24 hours cell lysate Lane 3: HeLa treated with 2mM thymidine for 16 hours then treated with 100nM Nocodazole for 24 hours cell lysate Lane 4: HT-29 cell lysate Lane 5: HT-29 treated with 100nM Nocodazole for 24 hours cell lysate Lane 6: HT-29 treated with 2mM thymidine for 16 hours then treated with 100nM Nocodazole for 24 hours cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 68 kDa Observed band size: 68 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1701-33) at 1/1,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded mouse thymus tissue with Rabbit anti-Phospho-PLK1 (T210) antibody (ET1701-33) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1701-33) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3: Flow cytometric analysis of Phospho-PLK1 (T210) was done on MCF-7 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1701-33, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.