GLUT2 Recombinant Rabbit Monoclonal Antibody [JJ20-21]
cat.: ET1701-34
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB, IHC-P, FC
Clonality: Monoclonal
Clone number: JJ20-21
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 57 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human Glucose Transporter GLUT2 aa 35-84 / 524.
Positive control: A549 cell lysates, human liver tissue, human kidney tissue, human pancreas tissue, HepG2.
Subcellular location: Membrane.
Recommended Dilutions:
  WB
  IHC-P
  FC
1:1,000
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: P11168 Human
Alternative names: liver Glucose Transporter 2 Glucose Transporter GLUT2 Glucose transporter type 2 Glucose transporter type 2 liver Glucose transporter, liver/islet GLUT-2 GLUT2 GTR2_HUMAN GTT2 SLC2A2 Solute carrier family 2 (facilitated glucose transporter) member 2 Solute carrier family 2 facilitated glucose transporter member 2 Solute carrier family 2, facilitated glucose transporter member 2
Images
ET1701-34_1.jpg Fig1: Western blot analysis of GLUT2 on A549 cell lysates with Rabbit anti-GLUT2 antibody (ET1701-34) at 1/1,000 dilution.

Lysates/proteins at 30 µg/Lane.

Predicted band size: 57 kDa
Observed band size: 50 kDa

Exposure time: 28 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1701-34) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
ET1701-34_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-GLUT2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1701-34, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1701-34_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-GLUT2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1701-34, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1701-34_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-GLUT2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1701-34, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1701-34_5.jpg Fig5: Flow cytometric analysis of GLUT2 was done on HepG2 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1701-34, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.