| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JJ20-21 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 57 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Glucose Transporter GLUT2 aa 35-84 / 524. |
| Positive control: | A549 cell lysate, PC-12 cell lysate, mouse liver tissue lysate, mouse small intestine tissue lysate, human liver tissue, human kidney tissue, human pancreas tissue, HepG2. |
| Subcellular location: | Membrane. |
| Recommended Dilutions:
WB |
1:2,000 |
| Uniprot #: | SwissProt: P11168 Human | P14246 Mouse | P12336 Rat |
| Alternative names: | liver Glucose Transporter 2 Glucose Transporter GLUT2 Glucose transporter type 2 Glucose transporter type 2 liver Glucose transporter, liver/islet GLUT-2 GLUT2 GTR2_HUMAN GTT2 SLC2A2 Solute carrier family 2 (facilitated glucose transporter) member 2 Solute carrier family 2 facilitated glucose transporter member 2 Solute carrier family 2, facilitated glucose transporter member 2 |
|
Fig1:
Western blot analysis of GLUT2 on different lysates with Rabbit anti-GLUT2 antibody (ET1701-34) at 1/2,000 dilution. Lane 1: A549 cell lysate (20 µg/Lane) Lane 2: PC-12 cell lysate (20 µg/Lane) Lane 3: Mouse liver tissue lysate (40 µg/Lane) Lane 4: Mouse small intestine tissue lysate (40 µg/Lane) Predicted band size: 57 kDa Observed band size: 57 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1701-34) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |