GLUT2 Recombinant Rabbit Monoclonal Antibody [JJ20-21]
cat.: ET1701-34
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB
Clonality: Monoclonal
Clone number: JJ20-21
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 57 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human Glucose Transporter GLUT2 aa 35-84 / 524.
Positive control: A549 cell lysate, PC-12 cell lysate, mouse liver tissue lysate, mouse small intestine tissue lysate, human liver tissue, human kidney tissue, human pancreas tissue, HepG2.
Subcellular location: Membrane.
Recommended Dilutions:
  WB

1:2,000
Uniprot #: SwissProt: P11168 Human | P14246 Mouse | P12336 Rat
Alternative names: liver Glucose Transporter 2 Glucose Transporter GLUT2 Glucose transporter type 2 Glucose transporter type 2 liver Glucose transporter, liver/islet GLUT-2 GLUT2 GTR2_HUMAN GTT2 SLC2A2 Solute carrier family 2 (facilitated glucose transporter) member 2 Solute carrier family 2 facilitated glucose transporter member 2 Solute carrier family 2, facilitated glucose transporter member 2
Images
ET1701-34_1.jpg Fig1: Western blot analysis of GLUT2 on different lysates with Rabbit anti-GLUT2 antibody (ET1701-34) at 1/2,000 dilution.

Lane 1: A549 cell lysate (20 µg/Lane)
Lane 2: PC-12 cell lysate (20 µg/Lane)
Lane 3: Mouse liver tissue lysate (40 µg/Lane)
Lane 4: Mouse small intestine tissue lysate (40 µg/Lane)

Predicted band size: 57 kDa
Observed band size: 57 kDa

Exposure time: 10 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1701-34) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.