Anti-PD-L1 antibody [JJ08-95]
cat.: ET1701-41
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB, IHC-P
Clonality: Monoclonal
Clone number: JJ08-95
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Protein A affinity purified.
Molecular weight: 33 kDa (Predicted band size)
Isotype: IgG
Immunogen: Synthetic peptide within Human PD-L1 aa 200-230 (Extracellular).
Positive control: A549 cell lysate, MCF-7 cell lysate, human non-small cell lung cancer tissue.
Subcellular location: Cell membrane, Endomembrane system.
Recommended Dilutions:
  WB
  IHC-P

1:500-1:2,000
1:50-1:200
Uniprot #: SwissProt: Q9NZQ7 Human | Q9EP73 Mouse
Alternative names: B7 H antibody B7 H1 antibody B7 homolog 1 antibody B7-H1 antibody B7H antibody B7H1 antibody CD 274 antibody CD274 antibody CD274 antigen antibody CD274 molecule antibody MGC142294 antibody MGC142296 antibody OTTHUMP00000021029 antibody PD L1 antibody PD-L1 antibody PD1L1_HUMAN antibody PDCD1 ligand 1 antibody PDCD1L1 antibody PDCD1LG1 antibody PDL 1 antibody PDL1 antibody Programmed cell death 1 ligand 1 antibody Programmed death ligand 1 antibody RGD1566211 antibody
Images
ET1701-41_1.jpg Fig1: Western blot analysis of PD-L1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1701-41, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: A549 cell lysates
Lane 2: MCF-7 cell lysates
ET1701-41_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded human non-small cell lung cancer tissue using anti-PD-L1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1701-41, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.