SIRT2 Recombinant Rabbit Monoclonal Antibody [JJ08-83]
cat.: ET1701-43
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JJ08-83 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 43 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human SIRT2 aa 1-50 / 389. |
| Positive control: | MCF7 cell lysate, HeLa cell lysate, HCT 116 cell lysate, human brain tissue lysate. |
| Subcellular location: | Cell membrane, Cell projection, Chromosome, Cytoplasm, Cytoskeleton, Membrane, Microtubule, Nucleus. |
| Recommended Dilutions:
WB |
1:1,000-1:2,000 |
| Uniprot #: | SwissProt: Q8IXJ6 Human |
| Alternative names: | FLJ35621 FLJ37491 NAD dependent deacetylase sirtuin 2 NAD-dependent deacetylase sirtuin-2 NAD-dependent protein deacetylase sirtuin-2 Regulatory protein SIR2 homolog 2 Silencing information regulator 2 like Silent information regulator 2 SIR2 SIR2 like protein 2 Sir2 related protein type 2 SIR2, S. cerevisiae, homolog-loke 2 SIR2-like protein 2 SIR2L SIR2L2 SIRT2 SIRT2_HUMAN Sirtuin (silent mating type information regulation 2 homolog) 2 (S.cerevisiae) Sirtuin 2 Sirtuin type 2 |
Images
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Fig1:
Western blot analysis of SIRT2 on different lysates with Rabbit anti-SIRT2 antibody (ET1701-43) at 1/1,000 dilution. Lane 1: MCF7 cell lysate (10 µg/Lane) Lane 2: HeLa cell lysate (10 µg/Lane) Lane 3: HCT 116 cell lysate (10 µg/Lane) Lane 4: Human brain tissue lysate (20 µg/Lane) Predicted band size: 43 kDa Observed band size: 40 kDa Exposure time: 1 minute; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1701-43) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of SIRT2 on different lysates with Rabbit anti-SIRT2 antibody (ET1701-43) at 1/2,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-SIRT2 KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 43 kDa Observed band size: 43 kDa Exposure time: 40 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1701-43) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.