JNK1+JNK3 Recombinant Rabbit Monoclonal Antibody [JJ08-86]
cat.: ET1701-47
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IP, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JJ08-86 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 48/53 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human JNK1 + JNK3 aa 1-423 / 427. |
| Positive control: | NIH/3T3 cell lysate, PC-12 cell lysate, human brain tissue. |
| Subcellular location: | Cytoplasm, Nucleus, Membrane, Mitochondrion. |
| Recommended Dilutions:
WB IP IHC-P |
1:1,000-1:2,000 Use at an assay dependent concentration. 1:200-1:1,000 |
| Uniprot #: | SwissProt: P45983 Human | P53779 Human | Q61831 Mouse | Q91Y86 Mouse | P49185 Rat | P49187 Rat |
| Alternative names: | c Jun N terminal kinase 3 c Jun N-terminal kinase 1 JNK 46 JNK JNK1 JNK1A2 JNK21B1/2 JNK3 alpha protein kinase JNK3 JNK3A JUN N terminal kinase MAP kinase 10 MAP kinase 8 MAP kinase p49 3F12 MAPK 10 MAPK10 MAPK8 Mitogen activated protein kinase 10 Mitogen activated protein kinase 8 Mitogen activated protein kinase 8 isoform JNK1 alpha1 Mitogen activated protein kinase 8 isoform JNK1 beta2 p493F12 p54bSAPK PRKM10 PRKM8 SAPK1 SAPK1b SAPK1c Stress activated protein kinase 1 Stress activated protein kinase 1b Stress activated protein kinase 1c Stress activated protein kinase beta Stress activated protein kinase JNK3 |
Images
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Fig1:
Western blot analysis of JNK1+JNK3 on different lysates with Rabbit anti-JNK1+JNK3 antibody (ET1701-47) at 1/2,000 dilution. Lane 1: Mouse brain Lane 2: Rat brain Lysates/proteins at 20 µg/Lane. Exposure time: 10 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ET1701-47, 1/2,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 48.3 kDa Observed band size: 53/48 kDa |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-JNK1+JNK3 antibody (ET1701-47) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1701-47) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.