JNK1+JNK3 Recombinant Rabbit Monoclonal Antibody [JJ08-86]
cat.: ET1701-47
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IP, IHC-P
Clonality: Monoclonal
Clone number: JJ08-86
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 48/53 kDa
Isotype: IgG
Immunogen: Recombinant protein within Human JNK1 + JNK3 aa 1-423 / 427.
Positive control: NIH/3T3 cell lysate, PC-12 cell lysate, human brain tissue.
Subcellular location: Cytoplasm, Nucleus, Membrane, Mitochondrion.
Recommended Dilutions:
  WB
  IP
  IHC-P

1:1,000-1:2,000
Use at an assay dependent concentration.
1:200-1:1,000
Uniprot #: SwissProt: P45983 Human | P53779 Human | Q61831 Mouse | Q91Y86 Mouse | P49185 Rat | P49187 Rat
Alternative names: c Jun N terminal kinase 3 c Jun N-terminal kinase 1 JNK 46 JNK JNK1 JNK1A2 JNK21B1/2 JNK3 alpha protein kinase JNK3 JNK3A JUN N terminal kinase MAP kinase 10 MAP kinase 8 MAP kinase p49 3F12 MAPK 10 MAPK10 MAPK8 Mitogen activated protein kinase 10 Mitogen activated protein kinase 8 Mitogen activated protein kinase 8 isoform JNK1 alpha1 Mitogen activated protein kinase 8 isoform JNK1 beta2 p493F12 p54bSAPK PRKM10 PRKM8 SAPK1 SAPK1b SAPK1c Stress activated protein kinase 1 Stress activated protein kinase 1b Stress activated protein kinase 1c Stress activated protein kinase beta Stress activated protein kinase JNK3
Images
ET1701-47_1.jpg Fig1: Western blot analysis of JNK1+JNK3 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1701-47, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: NIH/3T3 cell lysate
Lane 2: PC-12 cell lysate
ET1701-47_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-JNK1+JNK3 antibody (ET1701-47) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1701-47) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.