MEK3+MEK6 Recombinant Rabbit Monoclonal Antibody [JJ087-09]
cat.: ET1701-48
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IF-Tissue, IHC-P, FC, IP |
| Clonality: | Monoclonal |
| Clone number: | JJ087-09 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 39/37 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human MEK3+MEK6 aa 298-347 / 347. |
| Positive control: | Hela cell lysate, Jurkat cell lysate, Hela, HepG2, NIH/3T3, human colon tissue, mouse colon tissue. |
| Subcellular location: | Cytoplasm, Cytoskeleton, Nucleus. |
| Recommended Dilutions:
WB IF-Cell IF-Tissue IHC-P FC IP |
1:1,000-1:2,000 1:50-1:200 1:50-1:200 1:50-1:200 1:50-1:100 Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: P46734 Human | P52564 Human | O09110 Mouse | P70236 Mouse Entrez Gene: 114495 Rat | 303200 Rat |
| Alternative names: | Dual specificity mitogen activated protein kinase kinase 6 Dual specificity mitogen-activated protein kinase kinase 3 MAP kinase kinase 3 MAP kinase kinase 6 map2k3 MAP2K6 MAPK/ERK kinase 3 MAPK/ERK kinase 6 MAPKK 3 MAPKK 6 MAPKK3 MAPKK6 MEK 3 MEK 6 Mitogen activated protein kinase kinase 3 Mitogen activated protein kinase kinase 6 MKK3 MKK6 MP2K3_HUMAN PRKMK3 PRKMK6 Protein kinase, mitogen activated, kinase 6 (MAP kinase kinase 6) SAPK kinase 2 SAPKK 3 SAPKK-2 SAPKK2 SAPKK3 Stress activated protein kinase kinase 3 Stress-activated protein kinase kinase 2 |
Images
|
Fig1:
Western blot analysis of MEK3+MEK6 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1701-48, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Hela cell lysate Lane 2: Jurkat cell lysate |
|
Fig2: ICC staining of MEK3+MEK6 in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1701-48, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
|
Fig3: ICC staining of MEK3+MEK6 in HepG2 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1701-48, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
|
Fig4: ICC staining of MEK3+MEK6 in NIH/3T3 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1701-48, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
|
Fig5:
Immunohistochemical analysis of paraffin-embedded human colon tissue with Mouse anti-MEK3+MEK6 antibody (ET1701-48) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1701-48) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig6:
Immunohistochemical analysis of paraffin-embedded mouse colon tissue with Mouse anti-MEK3+MEK6 antibody (ET1701-48) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1701-48) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig7: Flow cytometric analysis of MEK3+MEK6 was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1701-48, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.