Phospho-SHIP (Y1020) Recombinant Rabbit Monoclonal Antibody [JJ082-03]
cat.: ET1701-5
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, IP |
| Clonality: | Monoclonal |
| Clone number: | JJ082-03 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 133 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic phospho-peptide corresponding to residues surrounding Tyr1020 of Human SHIP aa 991-1040 / 1,189. |
| Positive control: | A549, Hela, HepG2. |
| Subcellular location: | Cell membrane, Cytoplasm, Cytoskeleton, Membrane. |
| Recommended Dilutions:
WB IF-Cell IP |
1:1,000 1:50-1:200 Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: Q92835 Human |
| Alternative names: | Inositol polyphosphate 5 phosphatase of 145kDa 4 5-trisphosphate 5-phosphatase 1 hp51CN Inositol polyphosphate 5 phosphatase 145kDa Inositol polyphosphate 5 phosphatase Inositol polyphosphate-5-phosphatase of 145 kDa INPP5D MGC104855 MGC142140 MGC142142 p150Ship Phosphatidylinositol 3,4,5 trisphosphate 5 phosphatase 1 Phosphatidylinositol-3 SH2 containing inositol phosphatase isoform b SH2 domain containing inositol 5' phosphatase 1 SH2 domain containing inositol phosphatase 1 SH2 domain-containing inositol phosphatase 1 SH2 domain-containing inositol-5'-phosphatase 1 SHIP-1 SHIP1 SHIP1_HUMAN Signaling inositol polyphosphate 5 phosphatase SIP 145 SIP-145 SIP145 |
Images
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Fig1: ICC staining of Phospho-SHIP (Y1020) in A549 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1701-5, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
|
Fig2: ICC staining of Phospho-SHIP (Y1020) in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1701-5, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
|
Fig3: ICC staining of Phospho-SHIP (Y1020) in HepG2 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1701-5, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.