PAX8 Recombinant Rabbit Monoclonal Antibody [JJ08-88]
cat.: ET1701-50
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB, ICC/IF, IHC-P
Clonality: Monoclonal
Clone number: JJ08-88
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Protein A affinity purified.
Molecular weight: 48 kDa
Isotype: IgG
Immunogen: Recombinant protein within Human PAX8 aa 101-450 / 450.
Positive control: SKOV-3 cell lysates, SKOV-3, human thyroid tissue, human ovarian carcinoma tissue, human thyroid cancer tissue.
Subcellular location: Nucleus.
Recommended Dilutions:
  WB
  ICC/IF
  IHC-P

1:500-1:2,000
1:50-1:200
1:50-1:200
Uniprot #: SwissProt: Q06710 Human
Alternative names: OTTHUMP00000158659 OTTHUMP00000158660 OTTHUMP00000203723 OTTHUMP00000203724 Paired box 8 Paired box gene 8 paired box homeotic gene 8 Paired box protein Pax 8 Paired box protein Pax-8 Paired domain gene 8 PAX 8 PAX8 PAX8_HUMAN
Images
ET1701-50_1.jpg Fig1: Western blot analysis of PAX8 on SKOV-3 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1701-50, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
ET1701-50_2.jpg Fig2: ICC staining of PAX8 in SKOV-3 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1701-50, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1701-50_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human thyroid tissue using anti-PAX8 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1701-50, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1701-50_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma tissue using anti-PAX8 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1701-50, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1701-50_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue using anti-PAX8 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1701-50, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.