Caspase-10 Recombinant Rabbit Monoclonal Antibody [JJ0890]
cat.: ET1701-53
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P, FC, IP |
| Clonality: | Monoclonal |
| Clone number: | JJ0890 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 59 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human Caspase-10 aa 254-452 / 521. |
| Positive control: | K562 cell lysates, human liver carcinoma tissue, human kidney tissue, K562. |
| Subcellular location: | Cytosol. |
| Recommended Dilutions:
WB FC IHC-P IP |
1:1,000-1:2,000 1:50-1:100 1:50-1:200 Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: Q92851 Human |
| Alternative names: | Caspase10 ALPS2 Apoptotic protease Mch-4 CASP 10 CASP-10 CASP10 CASPA_HUMAN Caspase 10 apoptosis related cysteine peptidase Caspase-10 subunit p12 FADD like ICE2 Fas associated death domain protein FAS-associated death domain protein interleukin-1B-converting enzyme 2 FLICE 2 FLICE2 ICE like apoptotic protease 4 ICE-like apoptotic protease 4 Interleukin 1B converting enzyme 2 MCH 4 |
Images
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Fig1: Western blot analysis of Caspase-10 on K562 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1701-53, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Caspase-10 antibody (ET1701-53) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1701-53) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Caspase-10 antibody (ET1701-53) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1701-53) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4: Flow cytometric analysis of Caspase-10 was done on K562 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1701-53, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.