SOD2 Recombinant Rabbit Monoclonal Antibody [JJ089-02]
cat.: ET1701-54
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JJ089-02 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 25 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human SOD2 aa 1-50 / 222. |
| Positive control: | Mouse brain tissue lysate, SH-SY5Y cell lysate, mouse heart tissue lysate, human liver tissue, human kidney tissue, mouse liver tissue, mouse colon tissue, mouse heart tissue. |
| Subcellular location: | Mitochondrion matrix. |
| Recommended Dilutions:
WB IHC-P |
1:1,000-1:2,000 1:1,000-1:8,000 |
| Uniprot #: | SwissProt: P04179 Human | P09671 Mouse | P07895 Rat |
| Alternative names: | Indophenoloxidase B IPO B IPOB Manganese containing superoxide dismutase Manganese SOD Manganese superoxide dismutase Mangano superoxide dismutase Mn SOD Mn superoxide dismutase MNSOD MVCD6 SOD 2 SOD2 SODM_HUMAN Superoxide dismutase [Mn] mitochondrial Superoxide dismutase [Mn], mitochondrial Superoxide dismutase 2 mitochondrial |
Images
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Fig1:
Western blot analysis of SOD2 on different lysates with Rabbit anti-SOD2 antibody (ET1701-54) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: NIH/3T3 cell lysate Lane 3: Human liver tissue lysate Lane 4: Mouse liver tissue lysate Lane 5: Rat liver tissue lysate Cell lysates/proteins at 20 µg/Lane. Tissue lysates/proteins at 40 µg/Lane. Predicted band size: 25 kDa Observed band size: 23 kDa Exposure time: 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1701-54) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-SOD2 antibody (ET1701-54) at 1/8,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1701-54) at 1/8,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-SOD2 antibody (ET1701-54) at 1/4,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1701-54) at 1/4,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded rat liver tissue with Rabbit anti-SOD2 antibody (ET1701-54) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1701-54) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.