SOD2 Recombinant Rabbit Monoclonal Antibody [JJ089-02]
cat.: ET1701-54
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P
Clonality: Monoclonal
Clone number: JJ089-02
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 25 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human SOD2 aa 1-50 / 222.
Positive control: Mouse brain tissue lysate, SH-SY5Y cell lysate, mouse heart tissue lysate, human liver tissue, human kidney tissue, mouse liver tissue, mouse colon tissue, mouse heart tissue.
Subcellular location: Mitochondrion matrix.
Recommended Dilutions:
  WB
  IHC-P

1:1,000-1:2,000
1:50-1:200
Uniprot #: SwissProt: P04179 Human | P09671 Mouse | P07895 Rat
Alternative names: Indophenoloxidase B IPO B IPOB Manganese containing superoxide dismutase Manganese SOD Manganese superoxide dismutase Mangano superoxide dismutase Mn SOD Mn superoxide dismutase MNSOD MVCD6 SOD 2 SOD2 SODM_HUMAN Superoxide dismutase [Mn] mitochondrial Superoxide dismutase [Mn], mitochondrial Superoxide dismutase 2 mitochondrial
Images
ET1701-54_1.jpg Fig1: Western blot analysis of SOD2 on different lysates with Rabbit anti-SOD2 antibody (ET1701-54) at 1/500 dilution.

Lane 1: Mouse heart tissue lysate
Lane 2: SH-SY5Y cell lysate (10 µg/Lane)
Lane 3: Mouse brain tissue lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 25 kDa
Observed band size: 23 kDa

Exposure time: 1 minute;

12% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1701-54) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
ET1701-54_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-SOD2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1701-54, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1701-54_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-SOD2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1701-54, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1701-54_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-SOD2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1701-54, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1701-54_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-SOD2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1701-54, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1701-54_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-SOD2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1701-54, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1701-54_7.jpg Fig7: Western blot analysis of SOD2 on different lysates with Rabbit anti-SOD2 antibody (ET1701-54) at 1/1,000 dilution.

Lane 1: HeLa cell lysate
Lane 2: NIH/3T3 cell lysate
Lane 3: Human liver tissue lysate
Lane 4: Mouse liver tissue lysate
Lane 5: Rat liver tissue lysate

Cell lysates/proteins at 20 µg/Lane.
Tissue lysates/proteins at 40 µg/Lane.

Predicted band size: 25 kDa
Observed band size: 23 kDa

Exposure time: 10 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1701-54) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
ET1701-54_8.jpg Fig8: Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-SOD2 antibody (ET1701-54) at 1/8,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1701-54) at 1/8,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1701-54_9.jpg Fig9: Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-SOD2 antibody (ET1701-54) at 1/4,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1701-54) at 1/4,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1701-54_10.jpg Fig10: Immunohistochemical analysis of paraffin-embedded rat liver tissue with Rabbit anti-SOD2 antibody (ET1701-54) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1701-54) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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