KMT6/EZH2 Recombinant Rabbit Monoclonal Antibody [JJ089-9]
cat.: ET1701-56
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | IHC-P, IF-Cell, IF-Tissue |
| Clonality: | Monoclonal |
| Clone number: | JJ089-9 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 85 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human EZH2 aa 140-290. |
| Positive control: | Human breast cancer tissue, human colon cancer tissue, human tonsil tissue, human NK T-Cell lymphoma tissue, HeLa, Neuro-2a. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
IHC-P IF-Cell IF-Tissue |
1:500 1:100 1:50-1:200 |
| Uniprot #: | SwissProt: Q15910 Human | Q61188 Mouse |
| Alternative names: | Enhancer of zeste 2 enhancer of zeste 2 polycomb repressive complex 2 subunit Enhancer of zeste homolog 2 (Drosophila) Enhancer of zeste homolog 2 Enhancer of zeste, Drosophila, homolog 2 ENX 1 Enx 1h ENX-1 ENX1 Enx1h EZH 2 EZH1 EZH2 EZH2_HUMAN EZH2b Histone-lysine N-methyltransferase EZH2 KMT 6 KMT6 KMT6A Lysine N-methyltransferase 6 MGC9169 WVS WVS2 |
Images
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Fig1:
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Rabbit anti-KMT6/EZH2 antibody (ET1701-56) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1701-56) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-KMT6/EZH2 antibody (ET1701-56) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1701-56) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-KMT6/EZH2 antibody (ET1701-56) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1701-56) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded human NK T-Cell lymphoma tissue with Rabbit anti-KMT6/EZH2 antibody (ET1701-56) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1701-56) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunocytochemistry analysis of HeLa cells labeling KMT6/EZH2 with Rabbit anti-KMT6/EZH2 antibody (ET1701-56) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-KMT6/EZH2 antibody (ET1701-56) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig6:
Immunocytochemistry analysis of Neuro-2a cells labeling KMT6/EZH2 with Rabbit anti-KMT6/EZH2 antibody (ET1701-56) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-KMT6/EZH2 antibody (ET1701-56) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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