Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
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Species reactivity: | Human |
Applications: | WB, IF-Cell, IHC-P, FC, IP |
Clonality: | Monoclonal |
Clone number: | JJ08-97 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Protein A affinity purified. |
Molecular weight: | Predicted band size: 19 kDa |
Isotype: | IgG |
Immunogen: | Recombinant protein within Human CD3D aa 60-171 / 171. |
Positive control: | Jurkat cell lysates, Jurkat, human spleen tissue, human tonsil tissue. |
Subcellular location: | Membrane. |
Recommended Dilutions:
WB IF-Cell IHC-P FC IP |
1:1,000-1:2,000 1:100-1:500 1:50-1:200 1:50-1:100 Use at an assay dependent concentration. |
Uniprot #: | SwissProt: P04234 Human |
Alternative names: | CD3 antigen delta subunit CD3 delta CD3d CD3d antigen delta polypeptide (TiT3 complex) CD3d molecule delta (CD3-TCR complex) CD3D_HUMAN IMD19 OKT3 delta chain T cell receptor T3 delta chain T-cell receptor T3 delta chain T-cell surface glycoprotein CD3 delta chain T3D |
Fig1: Western blot analysis of CD3D on Jurkat cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1701-57, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. | |
Fig2: ICC staining of CD3D in Jurkat cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1701-57, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). | |
Fig3: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-CD3D antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1701-57, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. | |
Fig4: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD3D antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1701-57, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Fig5: Flow cytometric analysis of CD3D was done on Jurkat cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1701-57, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |