Anti-Integrin alpha 5 antibody [JJ08-94]
cat.: ET1701-58
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, ICC/IF, IHC-P, IP, FC
Clonality: Monoclonal
Clone number: JJ08-94
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Protein A affinity purified.
Molecular weight: 150 kDa
Isotype: IgG
Immunogen: Synthetic peptide within C-terminal human Integrin alpha 5.
Positive control: NIH/3T3 cell lysates, Hela, A549, human tonsil tissue, human kidney tissue, human uterus tissue, mouse uterus tissue, Jurkat.
Subcellular location: Membrane, Cell junction, Cell surface.
Recommended Dilutions:
  WB
  ICC/IF
  IHC-P
  FC
  IP

1:1,000-1:2,000
1:50-1:200
1:50-1:200
1:50-1:100
Use at an assay dependent concentration.
Uniprot #: SwissProt: P08648 Human | P11688 Mouse
Unigene: 100796 Rat
Alternative names: CD49 antigen-like family member E antibody CD49e antibody Fibronectin receptor subunit alpha antibody Fibronectin receptor, alpha subunit antibody FNRA antibody Integrin alpha 5 (fibronectin receptor alpha) antibody Integrin alpha-5 antibody Integrin alpha-5 light chain antibody Integrin alpha-F antibody Integrin, alpha 5 (fibronectin receptor, alpha polypeptide) antibody ITA5_HUMAN antibody Itga5 antibody Very late activation protein 5, alpha subunit antibody VLA-5 antibody VLA5 antibody VLA5A antibody
Images
ET1701-58_1.jpg Fig1: Western blot analysis of Integrin alpha 5 on NIH/3T3 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1701-58, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
ET1701-58_2.jpg Fig2: ICC staining of Integrin alpha 5 in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1701-58, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1701-58_3.jpg Fig3: ICC staining of Integrin alpha 5 in A549 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1701-58, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1701-58_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Integrin alpha 5 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1701-58, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1701-58_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Integrin alpha 5 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1701-58, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1701-58_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-Integrin alpha 5 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1701-58, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1701-58_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded mouse uterus tissue using anti-Integrin alpha 5 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1701-58, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1701-58_8.jpg Fig8: Flow cytometric analysis of Integrin alpha 5 was done on Jurkat cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1701-58, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
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