ARID1A Recombinant Rabbit Monoclonal Antibody [JJ09-01]
cat.: ET1701-60
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: IF-Cell, IF-Tissue, IHC-P
Clonality: Monoclonal
Clone number: JJ09-01
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 242 kDa
Isotype: IgG
Immunogen: Recombinant protein within Human ARID1A aa 1,184-1,334 / 2,285.
Positive control: SH-SY5Y, Hela, 293, human spleen tissue, mouse testis tissue, mouse colon tissue, mouse kidney tissue, rat testis tissue.
Subcellular location: Nucleus.
Recommended Dilutions:
  IF-Cell
  IF-Tissue
  IHC-P
1:50-1:200
1:50-1:200
1:50-1:400
Uniprot #: SwissProt: O14497 Human | A2BH40 Mouse
Unigene: 61077 Rat
Alternative names: actin-dependent regulator of chromatin subfamily F member 1 ARI1A_HUMAN ARID domain containing protein 1A ARID domain-containing protein 1A ARID1A AT rich interactive domain 1A (SWI like) AT rich interactive domain 1A AT rich interactive domain containing protein 1A AT-rich interactive domain-containing protein 1A B120 BAF250 BAF250A BM029 brain protein 120 BRG1 associated factor 250 BRG1 associated factor 250a BRG1-associated factor 250 BRG1-associated factor 250a C1ORF4 chromatin remodeling factor p250 chromosome 1 open reading frame 4 ELD hELD hOSA1 matrix-associated MRD14 Osa homolog 1 OSA1 OSA1 nuclear protein P270 SMARCF1 SWI like protein SWI SNF complex protein p270 SWI-like protein SWI/SNF complex protein p270 SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily f, member 1 SWI/SNF-related
Images
ET1701-60_1.jpg Fig1: ICC staining of ARID1A in SH-SY5Y cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1701-60, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1701-60_2.jpg Fig2: ICC staining of ARID1A in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1701-60, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1701-60_3.jpg Fig3: ICC staining of ARID1A in 293 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1701-60, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1701-60_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-ARID1A antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1701-60, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1701-60_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-ARID1A antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1701-60, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1701-60_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-ARID1A antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1701-60, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1701-60_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-ARID1A antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1701-60, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1701-60_8.jpg Fig8: Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-ARID1A antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1701-60, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.