ARID1A Recombinant Rabbit Monoclonal Antibody [JJ09-01]
cat.: ET1701-60
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | IF-Cell, IF-Tissue, IHC-P, WB |
| Clonality: | Monoclonal |
| Clone number: | JJ09-01 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 242 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human ARID1A aa 1,184-1,334 / 2,285. |
| Positive control: | K-562 cell lysate, AGS cell lysate, K-562, human colon tissue, mouse colon tissue, rat colon tissue. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
IF-Cell IF-Tissue IHC-P WB |
1:200-1:500 1:50-1:200 1:100-1:500 1:2,000 |
| Uniprot #: | SwissProt: O14497 Human | A2BH40 Mouse Unigene: 61077 Rat |
| Alternative names: | actin-dependent regulator of chromatin subfamily F member 1 ARI1A_HUMAN ARID domain containing protein 1A ARID domain-containing protein 1A ARID1A AT rich interactive domain 1A (SWI like) AT rich interactive domain 1A AT rich interactive domain containing protein 1A AT-rich interactive domain-containing protein 1A B120 BAF250 BAF250A BM029 brain protein 120 BRG1 associated factor 250 BRG1 associated factor 250a BRG1-associated factor 250 BRG1-associated factor 250a C1ORF4 chromatin remodeling factor p250 chromosome 1 open reading frame 4 ELD hELD hOSA1 matrix-associated MRD14 Osa homolog 1 OSA1 OSA1 nuclear protein P270 SMARCF1 SWI like protein SWI SNF complex protein p270 SWI-like protein SWI/SNF complex protein p270 SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily f, member 1 SWI/SNF-related |
Images
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Fig1:
Western blot analysis of ARID1A on different lysates with Rabbit anti-ARID1A antibody (ET1701-60) at 1/2,000 dilution. Lane 1: K-562 cell lysate (20 µg/Lane) Lane 2: AGS cell lysate (20 µg/Lane) Predicted band size: 242 kDa Observed band size: 150-300 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1701-60) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of K-562 cells labeling ARID1A with Rabbit anti-ARID1A antibody (ET1701-60) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-ARID1A antibody (ET1701-60) at 1/200 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-ARID1A antibody (ET1701-60) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1701-60) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded mouse colon tissue with Rabbit anti-ARID1A antibody (ET1701-60) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1701-60) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded rat colon tissue with Rabbit anti-ARID1A antibody (ET1701-60) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1701-60) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.