SUZ12 Recombinant Rabbit Monoclonal Antibody [JJ09-04]
cat.: ET1701-62
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IF-Tissue, IP |
| Clonality: | Monoclonal |
| Clone number: | JJ09-04 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 83 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human SUZ12 aa 1-150/739. |
| Positive control: | MCF-7 cells lysates, NIH/3T3 cell lysate, PC-12 cell lysate, Raw264.7 cell lysate, SW480, MCF-7, Hela, SKOV-3. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB IF-Cell IF-Tissue IP |
1:1,000-1:2,000 1:50-1:200 1:50-1:200 Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: Q15022 Human | Q80U70 Mouse Entrez Gene: 688041 Rat |
| Alternative names: | ChET 9 protein CHET9 Chromatin precipitated E2F target 9 protein JJAZ1 Joined to JAZF1 protein KIAA0160 Polycomb protein SUZ12 Suppressor of zeste 12 homolog Suppressor of zeste 12 protein homolog SUZ12 SUZ12 polycomb repressive complex 2 subunit SUZ12_HUMAN |
Images
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Fig1:
Western blot analysis of SUZ12 on different lysates with Rabbit anti-SUZ12 antibody (ET1701-62) at 1/500 dilution. Lane 1: NIH/3T3 cell lysate Lane 2: PC-12 cell lysate Lane 3: Raw264.7 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 83 kDa Observed band size: 83 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1701-62) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
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Fig2: Western blot analysis of SUZ12 on MCF-7 cells lysates using anti-SUZ12 antibody at 1/1,000 dilution. |
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Fig3: IF-cell staining SUZ12 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: IF-cell staining SUZ12 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig5: IF-cell staining SUZ12 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig6: IF-cell staining SUZ12 in SKOV-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.