COX IV Recombinant Rabbit Monoclonal Antibody [JJ09-05]
cat.: ET1701-63
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IF-Tissue, IHC-P, IP, FC(Intra) |
| Clonality: | Monoclonal |
| Clone number: | JJ09-05 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 20 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human COX IV aa 21-70 / 169. |
| Positive control: | HeLa cell lysate, HepG2 cell lysate, mouse heart tissue lysate, rat heart tissue lysate, HepG2, MCF-7, human liver tissue, human liver carcinoma tissue, human colon carcinoma tissue, human kidney tissue, mouse colon tissue, mouse heart tissue. |
| Subcellular location: | Mitochondrion inner membrane. |
| Recommended Dilutions:
WB IF-Cell IF-Tissue IHC-P IP FC(Intra) |
1:1,000-1:5,000 1:50-1:200 1:200 1:400-1:1,000 Use at an assay dependent concentration. 1:500-1:1,000 |
| Uniprot #: | SwissProt: P13073 Human | P19783 Mouse | P10888 Rat |
| Alternative names: | AL024441 COX 4 COX IV 1 COX IV COX IV-1 Cox4 COX41_HUMAN Cox4a COX4B COX4I1 COX4I2 COX4L2 COXIV Cytochrome c oxidase polypeptide IV Cytochrome c oxidase subunit 4 isoform 1 mitochondrial Cytochrome c oxidase subunit 4 isoform 1, mitochondrial Cytochrome C Oxidase subunit IV Cytochrome c oxidase subunit IV isoform 1 Cytochrome c oxidase subunit IV isoform 2 (lung) Cytochrome c oxydase subunit 4 dJ857M17.2 MGC105470 MGC72016 |
Images
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Fig1:
Western blot analysis of COX IV on different lysates with Rabbit anti-COX IV antibody (ET1701-63) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: HepG2 cell lysate (20 µg/Lane) Lane 3: Mouse heart tissue lysate (30 µg/Lane) Lane 4: Rat heart tissue lysate (30 µg/Lane) Predicted band size: 20 kDa Observed band size: 15 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1701-63) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of MCF-7 cells labeling COX IV with Rabbit anti-COX IV antibody (ET1701-63) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-COX IV antibody (ET1701-63) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue with Rabbit anti-COX IV antibody (ET1701-63) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1701-63) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-COX IV antibody (ET1701-63) at 1/800 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1701-63) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded mouse heart tissue with Rabbit anti-COX IV antibody (ET1701-63) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1701-63) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6:
Immunohistochemical analysis of paraffin-embedded rat heart tissue with Rabbit anti-COX IV antibody (ET1701-63) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1701-63) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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