HDAC6 Recombinant Rabbit Monoclonal Antibody [JJ09-09]
cat.: ET1701-66
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Monkey |
| Applications: | WB, IF-Tissue, IHC-P, IP |
| Clonality: | Monoclonal |
| Clone number: | JJ09-09 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 131 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human HDAC6 aa 1-50 / 1,215. |
| Positive control: | HeLa cell lysate, K-562 cell lysate, Jurkat cell lysate, COS-1 cell lysate, HCT 116 cell lysate, human kidney tissue, human breast tissue. |
| Subcellular location: | Cell projection, Cytoplasm, Cytoskeleton, Nucleus. |
| Recommended Dilutions:
WB IF-Tissue IHC-P IP |
1:1,000-1:5,000 1:50-1:200 1:50-1:400 Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: Q9UBN7 Human |
| Alternative names: | CPBHM FLJ16239 HD 6 HD6 HDAC 6 HDAC6 HDAC6_HUMAN Histone deacetylase 6 (HD6) Histone deacetylase 6 JM 21 JM21 KIAA0901 OTTHUMP00000032398 OTTHUMP00000197663 PPP1R90 Protein phosphatase 1 regulatory subunit 90 |
Images
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Fig1:
Western blot analysis of HDAC6 on different lysates with Rabbit anti-HDAC6 antibody (ET1701-66) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: K-562 cell lysate Lane 3: Jurkat cell lysate Lane 4: COS-1 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 131 kDa Observed band size: 160 kDa Exposure time: 25 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1701-66) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of HDAC6 on different lysates with Rabbit anti-HDAC6 antibody (ET1701-66) at 1/1,000 dilution. Lane 1: HCT 116-si NT cell lysate Lane 2: HCT 116-si HDAC6 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 131 kDa Observed band size: 160 kDa Exposure time: 20 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1701-66) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-HDAC6 antibody (ET1701-66) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1701-66) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded human breast tissue with Rabbit anti-HDAC6 antibody (ET1701-66) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1701-66) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.