Anti-FOXO4 antibody [JJ09-11]
cat.: ET1701-67
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, ICC/IF, IHC-P, FC, IP
Clonality: Monoclonal
Clone number: JJ09-11
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Protein A affinity purified.
Molecular weight: 54 kDa
Isotype: IgG
Immunogen: Recombinant protein within Human FOXO4 aa 456-505 / 505.
Positive control: 293T, Hela, HepG2, human placenta tissue, 293.
Subcellular location: Nucleus, Cytoplasm.
Recommended Dilutions:
  WB
  ICC/IF
  IHC-P
  FC
  IP

1:500
1:50-1:200
1:50-1:200
1:50-1:100
Use at an assay dependent concentration.
Uniprot #: SwissProt: P98177 Human | Q9WVH3 Mouse
Unigene: 19646 Rat
Alternative names: AFX AFX1 Afxh ALL1-fused gene from X chromosome Fork head domain transcription factor AFX1 Forkhead box O4 Forkhead box protein O4 FOXO 4 Foxo4 FOXO4_HUMAN MGC117660 MGC120490 Mixed lineage leukemia, translocated to, 7 MLLT7 Myeloid/lymphoid or mixed lineage leukemia (trithorax homolog, Drosophila); translocated to, 7 Myeloid/lymphoid or mixed lineage leukemia, translocated to, 7 RGD1561201
Images
ET1701-67_1.jpg Fig1: ICC staining of FOXO4 in 293T cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1701-67, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1701-67_2.jpg Fig2: ICC staining of FOXO4 in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1701-67, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1701-67_3.jpg Fig3: ICC staining of FOXO4 in HepG2 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1701-67, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1701-67_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-FOXO4 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1701-67, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1701-67_5.jpg Fig5: Flow cytometric analysis of FOXO4 was done on 293 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1701-67, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.