FOXO4 Recombinant Rabbit Monoclonal Antibody [JJ09-11]
cat.: ET1701-67
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Tissue, IHC-P, FC, IP |
| Clonality: | Monoclonal |
| Clone number: | JJ09-11 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 54 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human FOXO4 aa 456-505 / 505. |
| Positive control: | 293T, Hela, HepG2, human placenta tissue, 293. |
| Subcellular location: | Nucleus, Cytoplasm. |
| Recommended Dilutions:
WB IF-Tissue IHC-P FC IP |
1:500 1:50-1:200 1:50-1:200 1:1,000 Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: P98177 Human | Q9WVH3 Mouse Unigene: 19646 Rat |
| Alternative names: | AFX AFX1 Afxh ALL1-fused gene from X chromosome Fork head domain transcription factor AFX1 Forkhead box O4 Forkhead box protein O4 FOXO 4 Foxo4 FOXO4_HUMAN MGC117660 MGC120490 Mixed lineage leukemia, translocated to, 7 MLLT7 Myeloid/lymphoid or mixed lineage leukemia (trithorax homolog, Drosophila); translocated to, 7 Myeloid/lymphoid or mixed lineage leukemia, translocated to, 7 RGD1561201 |
Images
|
Fig1:
Western blot analysis of FOXO4 on different lysates with Rabbit anti-FOXO4 antibody (ET1701-67) at 1/500 dilution. Lane 1: HeLa cell lysate Lane 2: Mouse heart tissue lysate Lysates/proteins at 10 µg/Lane1 and 20 ug/Lane2. Predicted band size: 54 kDa Observed band size: 55 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1701-67) at 1/500 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human placenta tissue with Rabbit anti-FOXO4 antibody (ET1701-67) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1701-67) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Flow cytometric analysis of 293 cells labeling FOXO4. Cells were fixed and permeabilized. Then stained with the primary antibody (ET1701-67, 1:1000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.