Cdc25B Recombinant Rabbit Monoclonal Antibody [JJ091-2]
cat.: ET1701-68
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IP |
| Clonality: | Monoclonal |
| Clone number: | JJ091-2 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 65 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human Cdc25B aa 171-220 / 580. |
| Positive control: | MDA-MB-231 cell lysate, HT-29 cell lysate, HeLa cell lysate, HepG2 cell lysate. |
| Subcellular location: | Cytoplasm, Cytoskeleton. |
| Recommended Dilutions:
WB IP |
1:1,000-1:2,000 Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: P30305 Human |
| Alternative names: | AI604853 Cdc 25B Cdc25b CDC25HU2 Cdc25m2 Cell division cycle 25 homolog B Cell division cycle 25B Cell division cycle 25B isoform 1 Cell division cycle 25B isoform 2 Cell division cycle 25B isoform 3 Cell division cycle 25B isoform 4 Cell division cycle 25B isoform 5 Dual specificity phosphatase Cdc25B EC 3.1.3.48 M phase inducer phosphatase 2 M-phase inducer phosphatase 2 MPIP2_HUMAN |
Images
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Fig1:
Western blot analysis of Cdc25B on different lysates with Rabbit anti-Cdc25B antibody (ET1701-68) at 1/1,000 dilution. Lane 1: MDA-MB-231 cell lysate Lane 2: HT-29 cell lysate Lane 3: HeLa cell lysate Lane 4: HepG2 cell lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 65 kDa Observed band size: 65 kDa Exposure time: 6 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1701-68) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.