Cdc25B Recombinant Rabbit Monoclonal Antibody [JJ091-2]
cat.: ET1701-68
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, FC |
| Clonality: | Monoclonal |
| Clone number: | JJ091-2 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 65 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human Cdc25B aa 171-220 / 580. |
| Positive control: | HT-29 cell lysate, HeLa cell lysate, HepG2 cell lysate, U-937 cell lysate, HeLa. |
| Subcellular location: | Cytoplasm, Cytoskeleton. |
| Recommended Dilutions:
WB FC |
1:5,000 1:1,000 |
| Uniprot #: | SwissProt: P30305 Human |
| Alternative names: | AI604853 Cdc 25B Cdc25b CDC25HU2 Cdc25m2 Cell division cycle 25 homolog B Cell division cycle 25B Cell division cycle 25B isoform 1 Cell division cycle 25B isoform 2 Cell division cycle 25B isoform 3 Cell division cycle 25B isoform 4 Cell division cycle 25B isoform 5 Dual specificity phosphatase Cdc25B EC 3.1.3.48 M phase inducer phosphatase 2 M-phase inducer phosphatase 2 MPIP2_HUMAN |
Images
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Fig1:
Western blot analysis of Cdc25B on different lysates with Rabbit anti-Cdc25B antibody (ET1701-68) at 1/5,000 dilution. Lane 1: HT-29 cell lysate Lane 2: HeLa cell lysate Lane 3: HepG2 cell lysate Lane 4: U-937 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 65 kDa Observed band size: 55 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1701-68) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Flow cytometric analysis of HeLa cells labeling Cdc25B. Cells were fixed and permeabilized. Then stained with the primary antibody (ET1701-68, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.