Estrogen Receptor beta Recombinant Rabbit Monoclonal Antibody [JJ09-02]
cat.: ET1701-69
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JJ09-02 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 59 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human Estrogen Receptor beta aa 440-530 / 530. |
| Positive control: | MCF-7 cell lysate, Daudi cell lysate, human breast carcinoma tissue. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB IHC-P |
1:1,000-1:2,000 1:150 |
| Uniprot #: | SwissProt: Q92731 Human |
| Alternative names: | ER BETA ER-beta Erb ESR B ESR BETA ESR2 ESR2_HUMAN ESRB ESTRB estrogen nuclear receptor beta variant a estrogen nuclear receptor beta variant b estrogen receptor 2 (ER beta) Estrogen receptor 2 estrogen receptor beta 4 Estrogen receptor beta NR3A2 Nuclear receptor subfamily 3 group A member 2 |
Images
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Fig1:
Western blot analysis of Estrogen Receptor beta on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1701-69, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: MCF-7 cell lysate Lane 2: Daudi cell lysate |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Rabbit anti-Estrogen Receptor beta antibody (ET1701-69) at 1/150 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1701-69) at 1/150 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.