Estrogen Receptor beta Recombinant Rabbit Monoclonal Antibody [JJ09-02]
cat.: ET1701-69
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JJ09-02 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 59 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human Estrogen Receptor beta aa 440-530 / 530. |
| Positive control: | Daudi cell lysate, MDA-MB-468 cell lysate human breast carcinoma tissue. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB IHC-P |
1:1,000-1:2,000 1:150 |
| Uniprot #: | SwissProt: Q92731 Human |
| Alternative names: | ER BETA ER-beta Erb ESR B ESR BETA ESR2 ESR2_HUMAN ESRB ESTRB estrogen nuclear receptor beta variant a estrogen nuclear receptor beta variant b estrogen receptor 2 (ER beta) Estrogen receptor 2 estrogen receptor beta 4 Estrogen receptor beta NR3A2 Nuclear receptor subfamily 3 group A member 2 |
Images
|
Fig1:
Western blot analysis of Estrogen Receptor beta on different lysates with Rabbit anti-Estrogen Receptor beta antibody (ET1701-69) at 1/1,000 dilution. Lane 1: MDA-MB-231 cell lysate (low expression) Lane 2: Daudi cell lysate Lane 3: MDA-MB-468 cell lysate (low expression) Lane 4: SK-Br-3 cell lysate (low expression) Lane 5: MCF7 cell lysate (low expression) Lysates/proteins at 20 µg/Lane. Predicted band size: 59 kDa Observed band size: 55 kDa Exposure time: 1 minute; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1701-69) at 1/1,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Rabbit anti-Estrogen Receptor beta antibody (ET1701-69) at 1/150 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1701-69) at 1/150 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.