CDC42 Recombinant Rabbit Monoclonal Antibody [JJ086-04]
cat.: ET1701-7
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IP, FC |
| Clonality: | Monoclonal |
| Clone number: | JJ086-04 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 21 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human CDC42 aa 30-191 / 191. |
| Positive control: | A431 cell lysate, HepG2 cell lysate, MCF7 cell lysate, human breast carcinoma tissue, mouse pancreas tissue, Hela. |
| Subcellular location: | Cell membrane, Cell projection, Cytoplasm, Cytoskeleton, Membrane. |
| Recommended Dilutions:
WB FC IHC-P IP |
1:500-1:1,000 1:50-1:100 1:50-1:200 Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: P60953 Human | P60766 Mouse | Q8CFN2 Rat |
| Alternative names: | CDC42 CDC42_HUMAN CDC42Hs Cell division control protein 42 homolog Cell division cycle 42 (GTP binding protein 25kDa) Cell division cycle 42 dJ224A6.1.1 (cell division cycle 42 (GTP-binding protein, 25kD)) dJ224A6.1.2 (cell division cycle 42 (GTP-binding protein, 25kD)) G25K G25K GTP-binding protein Growth regulating protein GTP binding protein 25kDa Small GTP binding protein CDC42 TKS |
Images
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Fig1:
Western blot analysis of CDC42 on different lysates with Rabbit anti-CDC42 antibody (ET1701-7) at 1/1,000 dilution. Lane 1: A431 cell lysate Lane 2: HepG2 cell lysate Lane 3: MCF7 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 21 kDa Observed band size: 21 kDa Exposure time: 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1701-7) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-CDC42 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1701-7, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3: Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using anti-CDC42 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1701-7, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4: Flow cytometric analysis of CDC42 was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1701-7, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.