Anti-CDC42 antibody [JJ086-04]
cat.: ET1701-7
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P, IP, FC
Clonality: Monoclonal
Clone number: JJ086-04
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Protein A affinity purified.
Molecular weight: 21 kDa
Isotype: IgG
Immunogen: Recombinant protein within human CDC42 aa 30-191.
Positive control: Hela cell lysate, HepG2 cell lysate, Jurkat cell lysate, human breast carcinoma tissue, human pancreas tissue, mouse pancreas tissue, Hela.
Subcellular location: Cell membrane, Cytoplasm, Midbody.
Recommended Dilutions:
  WB
  FC
  IHC-P
  IP

1:500-1:1,000
1:50-1:100
1:50-1:200
Use at an assay dependent concentration.
Uniprot #: SwissProt: P60953 Human | P60766 Mouse | Q8CFN2 Rat
Alternative names: CDC42 antibody CDC42_HUMAN antibody CDC42Hs antibody Cell division control protein 42 homolog antibody Cell division cycle 42 (GTP binding protein 25kDa) antibody Cell division cycle 42 antibody dJ224A6.1.1 (cell division cycle 42 (GTP-binding protein, 25kD)) antibody dJ224A6.1.2 (cell division cycle 42 (GTP-binding protein, 25kD)) antibody G25K antibody G25K GTP-binding protein antibody Growth regulating protein antibody GTP binding protein 25kDa antibody Small GTP binding protein CDC42 antibody TKS antibody
Images
ET1701-7_1.jpg Fig1: Western blot analysis of CDC42 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1701-7, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Hela cell lysate
Lane 2: HepG2 cell lysate
Lane 3: Jurkat cell lysate
ET1701-7_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-CDC42 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1701-7, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1701-7_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-CDC42 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1701-7, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1701-7_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using anti-CDC42 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1701-7, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1701-7_5.jpg Fig5: Flow cytometric analysis of CDC42 was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1701-7, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.