mGluR2 Recombinant Rabbit Monoclonal Antibody [JJ091-09]
cat.: ET1701-74
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IF-Tissue, IHC-P
Clonality: Monoclonal
Clone number: JJ091-09
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 96 kDa
Isotype: IgG
Immunogen: Recombinant protein within human mGluR2 aa 750-872/872.
Positive control: SH-SY-5Y, Hela, SW480, mouse brain tissue, mouse brain tissue lysates, rat brain tissue.
Subcellular location: Cell membrane, Cell junction, Cell projection.
Recommended Dilutions:
  WB
  IF-Cell
  IF-Tissue
  IHC-P

1:500
1:50-1:200
1:50-1:200
1:50-1:200
Uniprot #: SwissProt: Q14416 Human | Q14BI2 Mouse | P31421 Rat
Alternative names: AMPA selective glutamate receptor 2 GLUR2 GLURB Glutamate metabotropic receptor 2 Glutamate receptor homolog Glutamate receptor metabotropic 2 GPRC1B GRM2 GRM2_HUMAN Metabotropic glutamate receptor 2 mGlu2 mGluR2 OTTHUMP00000210984 OTTHUMP00000210986
Images
ET1701-74_1.jpg Fig1: ICC staining mGluR2 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ET1701-74_2.jpg Fig2: ICC staining mGluR2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ET1701-74_3.jpg Fig3: ICC staining mGluR2 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ET1701-74_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-mGluR2 antibody (ET1701-74) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1701-74) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1701-74_5.jpg Fig5: Western blot analysis of mGluR2 on mouse brain tissue lysates with Rabbit anti-mGluR2 antibody (ET1701-74) at 1/500 dilution.

Lysates/proteins at 20 µg/Lane.

Predicted band size: 96 kDa
Observed band size: 110 kDa

Exposure time: 2 minutes;

8% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1701-74) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
ET1701-74_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-mGluR2 antibody (ET1701-74) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1701-74) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.