mGluR2 Recombinant Rabbit Monoclonal Antibody [JJ091-09]
cat.: ET1701-74
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IF-Tissue, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JJ091-09 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 96 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human mGluR2 aa 750-872/872. |
| Positive control: | SH-SY-5Y, Hela, SW480, mouse brain tissue, mouse brain tissue lysates, rat brain tissue. |
| Subcellular location: | Cell membrane, Cell junction, Cell projection. |
| Recommended Dilutions:
WB IF-Cell IF-Tissue IHC-P |
1:500 1:50-1:200 1:50-1:200 1:50-1:200 |
| Uniprot #: | SwissProt: Q14416 Human | Q14BI2 Mouse | P31421 Rat |
| Alternative names: | AMPA selective glutamate receptor 2 GLUR2 GLURB Glutamate metabotropic receptor 2 Glutamate receptor homolog Glutamate receptor metabotropic 2 GPRC1B GRM2 GRM2_HUMAN Metabotropic glutamate receptor 2 mGlu2 mGluR2 OTTHUMP00000210984 OTTHUMP00000210986 |
Images
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Fig1: ICC staining mGluR2 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig2: ICC staining mGluR2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining mGluR2 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-mGluR2 antibody (ET1701-74) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1701-74) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Western blot analysis of mGluR2 on mouse brain tissue lysates with Rabbit anti-mGluR2 antibody (ET1701-74) at 1/500 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 96 kDa Observed band size: 110 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1701-74) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
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Fig6:
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-mGluR2 antibody (ET1701-74) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1701-74) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.