Occludin Recombinant Rabbit Monoclonal Antibody [JJ091-08]
cat.: ET1701-76
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IP, FC, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | JJ091-08 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 59 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within N-terminal human Occludin. |
| Positive control: | MCF-7 cell lysate, Mouse smooth muscle tissue lysate, Caco-2. |
| Subcellular location: | Cell membrane, tight junction. |
| Recommended Dilutions:
WB FC IP IF-Cell |
1:1,000 1:1,000 Use at an assay dependent concentration. 1:500 |
| Uniprot #: | SwissProt: Q16625 Human | Q61146 Mouse |
| Alternative names: | BLCPMG FLJ08163 FLJ18079 FLJ77961 FLJ94056 MGC34277 Occludin Ocln OCLN_HUMAN Tight junction protein occludin |
Images
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Fig1:
Western blot analysis of Occludin on different lysates with Rabbit anti-Occludin antibody (ET1701-76) at 1/1,000 dilution. Lane 1: MCF-7 cell lysate (10 µg/Lane) Lane 2: Mouse smooth muscle tissue lysate (20 µg/Lane) Predicted band size: 59 kDa Observed band size: 59 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1701-76) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of Caco-2 cells labeling Occludin with Rabbit anti-Occludin antibody (ET1701-76) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Occludin antibody (ET1701-76) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig3:
Flow cytometric analysis of Caco-2 cells labeling Occludin. Cells were fixed and permeabilized. Then stained with the primary antibody (ET1701-76, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.