Occludin Recombinant Rabbit Monoclonal Antibody [JJ091-08]
cat.: ET1701-76
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IP, FC |
| Clonality: | Monoclonal |
| Clone number: | JJ091-08 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 59 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within N-terminal human Occludin. |
| Positive control: | MCF-7 cell lysate, mouse smooth muscle tissue lysate, 293. |
| Subcellular location: | Cell membrane, tight junction. |
| Recommended Dilutions:
WB FC IP |
1:1,000 1:50-1:100 Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: Q16625 Human | Q61146 Mouse |
| Alternative names: | BLCPMG FLJ08163 FLJ18079 FLJ77961 FLJ94056 MGC34277 Occludin Ocln OCLN_HUMAN Tight junction protein occludin |
Images
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Fig1:
Western blot analysis of Occludin on different lysates with Rabbit anti-Occludin antibody (ET1701-76) at 1/1,000 dilution. Lane 1: MCF-7 cell lysate (10 µg/Lane) Lane 2: Mouse smooth muscle tissue lysate (20 µg/Lane) Predicted band size: 59 kDa Observed band size: 59 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1701-76) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
|
Fig2: Flow cytometric analysis of Occludin was done on 293 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1701-76, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.