CXCL12/SDF1 Recombinant Rabbit Monoclonal Antibody [JJ09-20]
cat.: ET1701-77
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, IF-Tissue, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JJ09-20 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 11 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant full length protein of human SDF1. |
| Positive control: | Human kidney tissue. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
WB IF-Cell IF-Tissue IHC-P |
1:1,000-1:2,000 1:50-1:200 1:200 1:200 |
| Uniprot #: | SwissProt: P48061 Human |
| Alternative names: | 12-O-tetradecanoylphorbol 13-acetate repressed protein 1 AI174028 C-X-C motif chemokine 12 Chemokine (C-X-C motif) ligand 12 (stromal cell-derived factor 1) Chemokine (C-X-C motif) ligand 12 Chemokine CXC motif ligand 12 cxcl12 hIRH hSDF-1 Intercrine reduced in hepatomas IRH OTTHUMP00000019491 PBSF Pre-B cell growth-stimulating factor SCYB12 SDF 1 SDF-1 SDF-1-alpha(3-67) SDF-1a SDF-1b SDF1_HUMAN SDF1A SDF1B Stromal cell-derived factor 1 Stromal cell-derived factor 1 delta Stromal cell-derived factor 1 gamma Stromal cell-derived factor 1a Stromal cell-derived factor-1 alpha Thymic lymphoma cell-stimulating factor Tlsf TLSF-a TLSF-b Tlsfa Tlsfb TPAR1 |
Images
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Fig1:
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-CXCL12/SDF1 antibody (ET1701-77) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1701-77) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig2:
Immunofluorescence analysis of paraffin-embedded human kidney tissue labeling CXCL12/SDF1 with Rabbit anti-CXCL12/SDF1 antibody (ET1701-77) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (ET1701-77, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.