Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
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Species reactivity: | Mouse, Human, Rat |
Applications: | WB, IHC-P |
Clonality: | Monoclonal |
Clone number: | JJ0924 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Protein A affinity purified. |
Molecular weight: | Predicted band size: 24 kDa |
Isotype: | IgG |
Immunogen: | Synthetic peptide within Human ZNRF2 aa 162-206 / 242. |
Positive control: | Mouse testis tissue. |
Subcellular location: | Cell junction, Cell membrane, Cell projection, Endosome, Lysosome, Membrane, Synapse. |
Recommended Dilutions:
WB IHC-P |
1:1,500 1:50-1:200 |
Uniprot #: | SwissProt: Q8NHG8 Human | Q71FD5 Mouse Entrez Gene: 362367 Rat |
Alternative names: | E3 ubiquitin-protein ligase ZNRF2 Protein Ells2 RING finger protein 202 RNF202 Zinc/RING finger protein 2 Znrf2 ZNRF2_HUMAN |
Fig1: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-ZNRF2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1701-78, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |