RIP Recombinant Rabbit Monoclonal Antibody [JJ092-04]
cat.: ET1701-79
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, FC, IP |
| Clonality: | Monoclonal |
| Clone number: | JJ092-04 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 76 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human RIP aa 250-500. |
| Positive control: | HeLa cell lysates, Raji cell lysates, 293. |
| Subcellular location: | Cytoplasm, Cell membrane. |
| Recommended Dilutions:
WB FC IP |
1:1,000 1:1,000 1-2μg/sample |
| Uniprot #: | SwissProt: Q13546 Human |
| Alternative names: | Cell death protein RIP FLJ39204 OTTHUMP00000039163 Receptor (TNFRSF) interacting serine threonine kinase 1 receptor interacting protein 1 Receptor interacting protein Receptor interacting protein kinase 1 Receptor interacting serine threonine protein kinase 1 Receptor TNFRSF interacting serine threonine kinase 1 Receptor-interacting protein 1 Receptor-interacting serine/threonine-protein kinase 1 Rinp RIP 1 RIP Rip-1 RIP1 RIPK 1 Ripk1 RIPK1_HUMAN Serine threonine protein kinase RIP Serine/threonine-protein kinase RIP |
Images
|
Fig1:
Western blot analysis of RIP on different lysates with Rabbit anti-RIP antibody (ET1701-79) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: Raji cell lysate Lane 3: K-562 cell lysate Lane 4: 293T cell lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 76 kDa Observed band size: 75 kDa Exposure time: 2 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1701-79) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Flow cytometric analysis of HeLa cells labeling RIP. Cells were fixed and permeabilized. Then stained with the primary antibody (ET1701-79, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
|
Fig3:
RIP was immunoprecipitated from 0.2 mg HeLa cell lysate with ET1701-79 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using ET1701-79 at 1/1,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: ET1701-79 IP in HeLa cell lysate Lane 3: Rabbit IgG instead of ET1701-79 in HeLa cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 2 minutes; ECL: K1801 |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.