Actin Recombinant Rabbit Monoclonal Antibody [JJ09-29]
cat.: ET1701-80
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat, Zebrafish |
| Applications: | WB, IF-Cell, IF-Tissue, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JJ09-29 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 42 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Actin aa 45-80 / 377. |
| Positive control: | HeLa cell lysate, A431 cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, mouse brain tissue lysate, mouse colon tissue lysate, rat brain tissue lysate, rat colon tissue lysate, human colon carcinoma tissue, hybrid fish (crucian-carp) brain tissue lysate, hybrid fish (crucian-carp) kidney tissue lysate, NIH/3T3, mouse cardiac muscle tissue, mouse smooth muscle tissue. |
| Subcellular location: | Cytoplasm, Cytoskeleton. |
| Recommended Dilutions:
WB IF-Cell IF-Tissue IHC-P |
1:10,000-1:50,000 1:100-1:500 1:1,000 1:5,000 |
| Uniprot #: | SwissProt: P68133 Human | P68134 Mouse | P68136 Rat Entrez Gene: 407658 Zebrafish |
| Alternative names: | a actin ACTA ACTA1 Actin alpha skeletal muscle Actin actin, alpha 1, skeletal muscle 1 actin, alpha 1, skeletal muscle Actin, alpha skeletal muscle actina actine ACTS_HUMAN aktin Alpha Actin 1 Alpha skeletal muscle Actin alpha skeletal muscle alpha-actin Alpha-actin-1 ASMA CFTD CFTD1 CFTDM MPFD NEM1 NEM2 NEM3 nemaline myopathy type 3 |
Images
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Fig1:
Western blot analysis of Actin on different lysates with Rabbit anti-Actin antibody (ET1701-80) at 1/10,000 dilution. Lane 1: HeLa cell lysate (15 µg/Lane) Lane 2: A431 cell lysate (15 µg/Lane) Lane 3: NIH/3T3 cell lysate (15 µg/Lane) Lane 4: PC-12 cell lysate (15 µg/Lane) Lane 5: Mouse brain tissue lysate (20 µg/Lane) Lane 6: Mouse colon tissue lysate (20 µg/Lane) Lane 7: Rat brain tissue lysate (20 µg/Lane) Lane 8: Rat colon tissue lysate (20 µg/Lane) Predicted band size: 42 kDa Observed band size: 42 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1701-80) at 1/10,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/100,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Application: IF-tissue Species: Human Site: Colon carcinoma Sample: Paraffin-embedded section Antibody concentration: 1/100 |
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Fig3:
Western blot analysis of Actin on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1701-80, 1/10,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Hybrid fish (crucian-carp) brain tissue lysate Lane 2: Hybrid fish (crucian-carp) kidney tissue lysate |
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Fig4:
Immunocytochemistry analysis of NIH/3T3 cells labeling Actin with Rabbit anti-Actin antibody (ET1701-80) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Actin antibody (ET1701-80) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.