DUSP1 Recombinant Rabbit Monoclonal Antibody [JJ0930]
cat.: ET1701-82
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IP, FC, IF-Cell, IF-Tissue |
| Clonality: | Monoclonal |
| Clone number: | JJ0930 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 39 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human DUSP1 aa 112-156 / 367. |
| Positive control: | Jurkat cell lysate, SK-Br-3 cell lysate, NIH/3T3 cell lysate, mouse liver tissue lysate, mouse kidney tissue lysate, rat liver tissue lysate, rat kidney tissue lysate, NIH/3T3, C6, human lung cancer tissue, mouse lung tissue, rat lung tissue. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB IHC-P FC IP IF-Cell IF-Tissue |
1:500-1:2,000 1:1,000 1:1,000 1-2μg/sample 1:100 1:200 |
| Uniprot #: | SwissProt: P28562 Human | P28563 Mouse | Q64623 Rat |
| Alternative names: | CL 100 CL100 Dual Specificity Phosphatase 1 Dual specificity protein phosphatase 1 Dual specificity protein phosphatase hVH1 DUS1_HUMAN DUSP 1 Dusp1 HVH1 MAP kinase phosphatase 1 Mitogen-activated protein kinase phosphatase 1 MKP-1 MKP1 Protein tyrosine phosphatase CL100 Protein-tyrosine phosphatase CL100 PTPN10 Serine/threonine specific protein phosphatase VH1 |
Images
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Fig1:
Western blot analysis of DUSP1 on different lysates with Rabbit anti-DUSP1 antibody (ET1701-82) at 1/2,000 dilution. Lane 1: Jurkat cell lysate Lane 2: SK-Br-3 cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: Mouse liver tissue lysate Lane 5: Mouse kidney tissue lysate Lane 6: Rat liver tissue lysate Lane 7: Rat kidney tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 39 kDa Observed band size: 50 kDa Exposure time: 5 minutes 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1701-82) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of NIH/3T3 cells labeling DUSP1 with Rabbit anti-DUSP1 antibody (ET1701-82) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-DUSP1 antibody (ET1701-82) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig3:
Immunocytochemistry analysis of C6 cells labeling DUSP1 with Rabbit anti-DUSP1 antibody (ET1701-82) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-DUSP1 antibody (ET1701-82) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue with Rabbit anti-DUSP1 antibody (ET1701-82) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1701-82) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded mouse lung tissue with Rabbit anti-DUSP1 antibody (ET1701-82) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1701-82) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6:
Immunohistochemical analysis of paraffin-embedded rat lung tissue with Rabbit anti-DUSP1 antibody (ET1701-82) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1701-82) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig7:
DUSP1 was immunoprecipitated from 0.2 mg SK-Br-3 cell lysate with ET1701-82 at 2 µg/25 µl agarose. Western blot was performed from the immunoprecipitate using ET1701-82 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: SK-Br-3 cell lysate (input) Lane 2: ET1701-82 IP in SK-Br-3 cell lysate Lane 3: Rabbit IgG instead of ET1701-82 in SK-Br-3 cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 41 seconds; ECL: K1802 |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.