| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IP |
| Clonality: | Monoclonal |
| Clone number: | JJ092-07 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 22 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within C-terminal human Glutathione Peroxidase 1. |
| Positive control: | HepG2 cell lysate. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB IP |
1:500-1:2,000 Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: P07203 Human | P11352 Mouse | P04041 Rat |
| Alternative names: | AL033363 Cellular glutathione peroxidase Glutathione peroxidase 1 Glutathione peroxidase GPx 1 GPx-1 GPX1 GPX1_HUMAN GPXD GSHPx-1 GSHPX1 MGC14399 MGC88245 |
|
Fig1:
Western blot analysis of Glutathione Peroxidase 1 on different lysates with Rabbit anti-Glutathione Peroxidase 1 antibody (ET1701-84) at 1/1,000 dilution. Lane 1: Hep G2 (Human hepatocellular carcinoma cell) cell lysate Lysates/proteins at 10 µg/Lane. Exposure time: 25 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ET1701-84, 1/1,000 in 5% NFDM/TBST, overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 22.1 kDa Observed band size: 22 kDa |