Glutathione Peroxidase 1 Recombinant Rabbit Monoclonal Antibody [JJ092-07]
cat.: ET1701-84
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IP
Clonality: Monoclonal
Clone number: JJ092-07
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: 22 kDa
Isotype: IgG
Immunogen: Synthetic peptide within C-terminal human Glutathione Peroxidase 1.
Positive control: HepG2 cell lysate.
Subcellular location: Cytoplasm.
Recommended Dilutions:
  WB
  IP

1:500-1:2,000
Use at an assay dependent concentration.
Uniprot #: SwissProt: P07203 Human | P11352 Mouse | P04041 Rat
Alternative names: AL033363 Cellular glutathione peroxidase Glutathione peroxidase 1 Glutathione peroxidase GPx 1 GPx-1 GPX1 GPX1_HUMAN GPXD GSHPx-1 GSHPX1 MGC14399 MGC88245
Images
ET1701-84_1.jpg Fig1: Western blot analysis of Glutathione Peroxidase 1 on different lysates with Rabbit anti-Glutathione Peroxidase 1 antibody (ET1701-84) at 1/1,000 dilution.

Lane 1: Hep G2 (Human hepatocellular carcinoma cell) cell lysate

Lysates/proteins at 10 µg/Lane.
Exposure time: 25 seconds; ECL: K1801

Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: ET1701-84, 1/1,000 in 5% NFDM/TBST, overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

Predicted band size: 22.1 kDa
Observed band size: 22 kDa
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.