FUS/TLS Recombinant Rabbit Monoclonal Antibody [JJ09-31]
cat.: ET1701-86
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse
Applications: WB, IHC-P, FC, IF-Cell
Clonality: Monoclonal
Clone number: JJ09-31
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 53 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human aa 1-46 / 526.
Positive control: K562 cell lysates, HepG2, NIH/3T3, human tonsil tissue, human colon carcinoma tissue, mouse brain tissue.
Subcellular location: Nucleus.
Recommended Dilutions:
  WB
  IHC-P
  FC
  IF-Cell
1:500-1:5,000
1:50-1:200
1:1,000
1:100
Uniprot #: SwissProt: P35637 Human | P56959 Mouse
Alternative names: 75 kDa DNA pairing protein 75 kDa DNA-pairing protein ALS6 Amyotrophic lateral sclerosis 6 fus FUS CHOP Fus like protein FUS_HUMAN FUS1 Fused in sarcoma Fusion (involved in t(12;16) in malignant liposarcoma) Fusion derived from t(12;16) malignant liposarcoma Fusion gene in myxoid liposarcoma Heterogeneous nuclear ribonucleoprotein P2 hnRNP P2 hnRNPP2 Oncogene FUS Oncogene TLS POMp75 RNA binding protein FUS RNA-binding protein FUS TLS TLS CHOP Translocated in liposarcoma Translocated in liposarcoma protein
Images
ET1701-86_1.jpg Fig1: Western blot analysis of FUS/TLS on different lysates with Rabbit anti-FUS/TLS antibody (ET1701-86) at 1/1,000 dilution.

Lane 1: HepG2 cell lysate
Lane 2: K-562 cell lysate
Lane 3: SH-SY5Y cell lysate
Lane 4: NIH/3T3 cell lysate
Lane 5: COS-1 cell lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 50 kDa
Observed band size: 70 kDa

Exposure time: 1 minutes 20 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1701-86) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
ET1701-86_2.jpg Fig2: Immunocytochemistry analysis of HepG2 cells labeling FUS/TLS with Rabbit anti-FUS/TLS antibody (ET1701-86) at 1/100 dilution.

Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-FUS/TLS antibody (ET1701-86) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
ET1701-86_3.jpg Fig3: Immunocytochemistry analysis of NIH/3T3 cells labeling FUS/TLS with Rabbit anti-FUS/TLS antibody (ET1701-86) at 1/100 dilution.

Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-FUS/TLS antibody (ET1701-86) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
ET1701-86_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-FUS/TLS antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1701-86, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1701-86_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-FUS/TLS antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1701-86, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1701-86_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-FUS/TLS antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1701-86, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1701-86_7.jpg Fig7: Flow cytometric analysis of HepG2 cells labeling FUS/TLS.

Cells were fixed and permeabilized. Then stained with the primary antibody (ET1701-86, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
ET1701-86_8.jpg Fig8: Flow cytometric analysis of NIH/3T3 cells labeling FUS/TLS.

Cells were fixed and permeabilized. Then stained with the primary antibody (ET1701-86, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.