Von Willebrand Factor Recombinant Rabbit Monoclonal Antibody [JJ09-34]
cat.: ET1701-87
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | JJ09-34 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 309 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human Von Willebrand Factor aa 1170-1320 / 2813. |
| Positive control: | bEnd.3 cell lysate, Human lung tissue lysate, Mouse lung tissue lysate, Mouse liver tissue lysate, human tonsil tissue, bEnd.3. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
WB IHC-P FC |
1:1,000-1:5,000 1:50-1:200 1:1,000 |
| Uniprot #: | SwissProt: P04275 Human | Q8CIZ8 Mouse |
| Alternative names: | Coagulation factor VIII Coagulation factor VIII VWF F8VWF Factor VIII related antigen von Willebrand antigen 2 von Willebrand antigen II Von Willebrand disease VWD vWF VWF_HUMAN |
Images
|
Fig1:
Western blot analysis of Von Willebrand Factor on different lysates with Rabbit anti-Von Willebrand Factor antibody (ET1701-87) at 1/1,000 dilution. Lane 1: bEnd.3 cell lysate Lane 2: Human lung tissue lysate Lane 3: Mouse lung tissue lysate Lane 4: Mouse liver tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 309 kDa Observed band size: 309 kDa Exposure time: 5 minutes; 3-8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1701-87) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-Von Willebrand Factor antibody (ET1701-87) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1701-87) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Flow cytometric analysis of bEnd.3 cells labeling Von Willebrand Factor. Cells were fixed and permeabilized. Then stained with the primary antibody (ET1701-87, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.