VE Cadherin Recombinant Rabbit Monoclonal Antibody [JJ0941]
cat.: ET1701-93
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse |
| Applications: | WB, IP |
| Clonality: | Monoclonal |
| Clone number: | JJ0941 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 88 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within mouse VE Cadherin aa 57-254 / 784. |
| Positive control: | BEnd.3 (Mouse brain microvascular endothelial cell) cell lysate, Mouse lung tissue lysate, Mouse skeletal muscle tissue lysate, Mouse heart tissue lysate, Mouse kidney tissue lysate, Mouse spleen tissue lysate. |
| Subcellular location: | Cell junction, adherens junction, Cell membrane, Cytoplasm. |
| Recommended Dilutions:
WB |
1:5,000 |
| Uniprot #: | SwissProt: P55284 Mouse |
| Alternative names: | 7B 4 7B4 7B4 antigen CADH5_HUMAN Cadherin 5 Cadherin 5 type 2 Cadherin 5, type 2 (vascular endothelium) Cadherin 5, type 2, VE cadherin (vascular epithelium) cadherin, vascular endothelial cadherin, vascular endothelial, 1 Cadherin-5 Cadherin5 CD 144 CD144 CD144 antigen CDH 5 CDH5 CDH5 protein Endothelial specific cadherin FLJ17376 OTTHUMP00000174777 Vascular endothelial cadherin Vascular epithelium cadherin VE Cad VE-cadherin VEC |
Images
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Fig1:
Western blot analysis of VE Cadherin on different lysates with Rabbit anti-VE Cadherin antibody (ET1701-93) at 1/5,000 dilution. Lane 1: bEnd.3 (Mouse brain microvascular endothelial cell) cell lysate Lane 2: NIH/3T3 (Mouse fibroblast) cell lysate Lysates/proteins at 15 µg/Lane. Exposure time: 2 minutes; ECL: K1801 Low expression of VE Cadherin protein in NIH/3T3 cell is consistent with the predicted expression pattern. Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ET1701-93, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 88 kDa Observed band size: 90-150 kDa |
|
Fig2:
Western blot analysis of VE Cadherin on different lysates with Rabbit anti-VE Cadherin antibody (ET1701-93) at 1/5,000 dilution. Lane 1: Mouse lung tissue lysate Lane 2: Mouse skeletal muscle tissue lysate Lane 3: Mouse heart tissue lysate Lane 4: Mouse kidney tissue lysate Lane 5: Mouse spleen tissue lysate Lysates/proteins at 30 µg/Lane. Exposure time: 2 minutes; ECL: K1801 The molecular weight observed is consistent with what has been described in the literature (PMID: 36631513). Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ET1701-93, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 88 kDa Observed band size: 90-150 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.