| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse |
| Applications: | WB, IP |
| Clonality: | Monoclonal |
| Clone number: | JJ0941 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 88 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within mouse VE Cadherin aa 57-254 / 784. |
| Positive control: | Mouse heart tissue lysate, mouse placenta tissue lysate, mouse spleen tissue lysate, mouse lung tissue lysate. |
| Subcellular location: | Cell junction, Cell membrane. |
| Recommended Dilutions:
WB |
1:1,000-1:5,000 |
| Uniprot #: | SwissProt: P55284 Mouse |
| Alternative names: | 7B 4 7B4 7B4 antigen CADH5_HUMAN Cadherin 5 Cadherin 5 type 2 Cadherin 5, type 2 (vascular endothelium) Cadherin 5, type 2, VE cadherin (vascular epithelium) cadherin, vascular endothelial cadherin, vascular endothelial, 1 Cadherin-5 Cadherin5 CD 144 CD144 CD144 antigen CDH 5 CDH5 CDH5 protein Endothelial specific cadherin FLJ17376 OTTHUMP00000174777 Vascular endothelial cadherin Vascular epithelium cadherin VE Cad VE-cadherin VEC |
|
Fig1:
Western blot analysis of VE Cadherin on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1701-93, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Mouse heart tissue lysate Lane 2: Mouse placenta tissue lysate Lane 3: Mouse spleen tissue lysate Lane 4: Mouse lung tissue lysate |