c-Fos Recombinant Rabbit Monoclonal Antibody [JJ0938]
cat.: ET1701-95
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse, Human, Rat |
| Applications: | WB, IHC-P, FC, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | JJ0938 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 41 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human c-Fos aa 231-268 / 380. |
| Positive control: | Human placenta tissue, NIH/3T3, NIH/3T3 cell lysate, MCF-7 cell lysate, HeLa serum starved for 40 hours then treated with 20% FBS for 2 hours. |
| Subcellular location: | Nucleus, Endoplasmic reticulum, Cytoplasm. |
| Recommended Dilutions:
WB IHC-P FC |
1:500 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: P01100 Human | P01101 Mouse | P12841 Rat |
| Alternative names: | Activator protein 1 AP 1 C FOS Cellular oncogene c fos Cellular oncogene fos FBJ murine osteosarcoma viral (v fos) oncogene homolog (oncogene FOS) FBJ murine osteosarcoma viral oncogene homolog FBJ murine osteosarcoma viral v fos oncogene homolog FBJ Osteosarcoma Virus FOS FOS protein FOS_HUMAN G0 G1 switch regulatory protein 7 G0/G1 switch regulatory protein 7 G0S7 Oncogene FOS p55 proto oncogene c Fos Proto oncogene protein c fos Proto-oncogene c-Fos v fos FBJ murine osteosarcoma viral oncogene homolog |
Images
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Fig1:
Western blot analysis of c-Fos on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1701-95, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: NIH/3T3 cell lysate Lane 2: MCF-7 cell lysate |
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Fig2: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-c-Fos antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1701-95, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3: Flow cytometric analysis of c-Fos was done on NIH/3T3 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1701-95, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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Fig4:
Immunocytochemistry analysis of normal HeLa / HeLa serum starved for 40 hours then treated with 20% FBS for 2 hours cells labeling c-Fos with Rabbit anti-c-Fos antibody (ET1701-95) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-c-Fos antibody (ET1701-95) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.