EBP50 Recombinant Rabbit Monoclonal Antibody [JJ0946]
cat.: ET1701-96
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JJ0946 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 39 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human EBP50 aa 1-150 / 358. |
| Positive control: | MCF-7, rat kidney tissue, human kidney tissue. |
| Subcellular location: | Cytoplasm, Apical cell membrane, Endomembrane system, Cell projection. |
| Recommended Dilutions:
WB IHC-P |
1:1,000-1:2,000 1:50-1:200 |
| Uniprot #: | SwissProt: O14745 Human | Q9JJ19 Rat |
| Alternative names: | EBP 50 EBP50 Ezrin radixin moesin binding phosphoprotein 50 Ezrin-radixin-moesin-binding phosphoprotein 50 Na(+)/H(+) exchange regulatory cofactor NHE RF Na(+)/H(+) exchange regulatory cofactor NHE-RF1 Na+/H+ exchange regulatory co factor NHERF 1 NHERF NHERF-1 NHERF1 NHRF1_HUMAN NPHLOP2 Regulatory cofactor of Na(+)/H(+) exchanger Slc9a3r1 Sodium hydrogen exchanger regulatory factor 1 Sodium-hydrogen exchanger regulatory factor 1 Sodium/hydrogen exchanger regulatory factor 1 Solute carrier family 9 (sodium/hydrogen exchanger) member 3 regulator 1 Solute carrier family 9 (sodium/hydrogen exchanger), isoform 3 regulatory factor 1 Solute carrier family 9 isoform 3 regulatory factor 1 Solute carrier family 9 isoform A3 regulatory factor 1 Solute carrier family 9 member 3 regulator 1 |
Images
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Fig1:
Western blot analysis of EBP50 on MCF-7 cell lysates with Rabbit anti-EBP50 antibody (ET1701-96) at 1/1000 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 38 kDa Observed band size: 50 kDa Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1701-96) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-EBP50 antibody (ET1701-96) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1701-96) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-EBP50 antibody (ET1701-96) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1701-96) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.