EBP50 Recombinant Rabbit Monoclonal Antibody [JJ0946]
cat.: ET1701-96
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Rat
Applications: WB, IHC-P
Clonality: Monoclonal
Clone number: JJ0946
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 39 kDa
Isotype: IgG
Immunogen: Recombinant protein within Human EBP50 aa 1-150 / 358.
Positive control: MCF-7, rat kidney tissue, human kidney tissue.
Subcellular location: Cytoplasm, Apical cell membrane, Endomembrane system, Cell projection.
Recommended Dilutions:
  WB
  IHC-P

1:1,000-1:2,000
1:50-1:200
Uniprot #: SwissProt: O14745 Human | Q9JJ19 Rat
Alternative names: EBP 50 EBP50 Ezrin radixin moesin binding phosphoprotein 50 Ezrin-radixin-moesin-binding phosphoprotein 50 Na(+)/H(+) exchange regulatory cofactor NHE RF Na(+)/H(+) exchange regulatory cofactor NHE-RF1 Na+/H+ exchange regulatory co factor NHERF 1 NHERF NHERF-1 NHERF1 NHRF1_HUMAN NPHLOP2 Regulatory cofactor of Na(+)/H(+) exchanger Slc9a3r1 Sodium hydrogen exchanger regulatory factor 1 Sodium-hydrogen exchanger regulatory factor 1 Sodium/hydrogen exchanger regulatory factor 1 Solute carrier family 9 (sodium/hydrogen exchanger) member 3 regulator 1 Solute carrier family 9 (sodium/hydrogen exchanger), isoform 3 regulatory factor 1 Solute carrier family 9 isoform 3 regulatory factor 1 Solute carrier family 9 isoform A3 regulatory factor 1 Solute carrier family 9 member 3 regulator 1
Images
ET1701-96_1.jpg Fig1: Western blot analysis of EBP50 on MCF-7 cell lysates with Rabbit anti-EBP50 antibody (ET1701-96) at 1/1000 dilution.

Lysates/proteins at 10 µg/Lane.

Predicted band size: 38 kDa
Observed band size: 50 kDa


Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1701-96) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
ET1701-96_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-EBP50 antibody (ET1701-96) at 1/50 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1701-96) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1701-96_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-EBP50 antibody (ET1701-96) at 1/50 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1701-96) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.