Doublecortin Recombinant Rabbit Monoclonal Antibody [JJ0959]
cat.: ET1701-98
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat, Cynomolgus monkey, Pig |
| Applications: | WB, IHC-P, IHC-Fr, IF-Tissue |
| Clonality: | Monoclonal |
| Clone number: | JJ0959 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 41 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Doublecortin aa 22-71 / 365. |
| Positive control: | Neuro-2a cell lysate, human brain tissue lysate, mouse brain tissue lysate, rat brain tissue lysate, HepG2. |
| Subcellular location: | Cytoplasm, Cell projection, neuron projection. |
| Recommended Dilutions:
WB IHC-P IHC-Fr IF-Tissue |
1:1,000-1:5,000 1:1,000 1:500 1:200-1:500 |
| Uniprot #: | SwissProt: O43602 Human | O88809 Mouse | Q9ESI7 Rat |
| Alternative names: | DBCN Dbct DC DCX DCX_HUMAN Doublecortex Doublin FLJ51296 Lis X Lis-X Lissencephalin X Lissencephalin-X Lissencephaly X linked Lissencephaly X linked doublecortin LISX Neuronal migration protein doublecortin OTTHUMP00000023859 OTTHUMP00000023860 OTTHUMP00000216315 OTTHUMP00000216316 SCLH XLIS |
Images
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Fig1:
Application: IHC-Fr Species: Mouse Site: Hippocampus Sample: Frozen section Antibody concentration: 1:500 Antigen retrieval: Not required |
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Fig2:
Application: IHC-Fr Species: Rat Site: Hippocampus Sample: Frozen section Antibody concentration: 1:500 Antigen retrieval: Not required |
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Fig3:
Western blot analysis of Doublecortin on different lysates with Rabbit anti-Doublecortin antibody (ET1701-98) at 1/1,000 dilution. Lane 1: Neuro-2a cell lysate (20 µg/Lane) Lane 2: Human brain tissue lysate (40 µg/Lane) Lane 3: Mouse brain tissue lysate (40 µg/Lane) Lane 4: Rat brain tissue lysate (40 µg/Lane) Predicted band size: 41 kDa Observed band size: 41/45 kDa Exposure time: 1 minute; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1701-98) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue with Rabbit anti-Doublecortin antibody (ET1701-98) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1701-98) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded rat hippocampus tissue with Rabbit anti-Doublecortin antibody (ET1701-98) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1701-98) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6:
Application: IF-Tissue Species: Mouse Site: Hippocampus Sample: Paraffin-embedded section Antibody concentration: 1/500 |
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Fig7:
Application: IF-Tissue Species: Rat Site: Hippocampus Sample: Paraffin-embedded section Antibody concentration: 1/500 |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.