Laminin 5 alpha 3 Recombinant Rabbit Monoclonal Antibody [JJ0957]
cat.: ET1701-99
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | JJ0957 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 367 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human Laminin 5 alpha 3 aa 50-150. |
| Positive control: | A431 cell lysates, Hela, HepG2. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
WB IF-Cell |
1:500-1:1,000 1:100-1:500 |
| Uniprot #: | SwissProt: Q16787 Human |
| Alternative names: | E170 Epiligrin 170 kDa subunit Epiligrin subunit alpha Kalinin subunit alpha LAMA3 LAMA3A Laminin 5 alpha3 Laminin A3 laminin alpha 3 laminin alpha3 Laminin subunit alpha-3 Laminin-5 subunit alpha Laminin-6 subunit alpha Laminin-7 subunit alpha laminin5 alpha 3 Laminin5 alpha3 LAMNA LOCS Nicein subunit alpha |
Images
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Fig1: ICC staining of Laminin 5 alpha 3 in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1701-99, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
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Fig2: ICC staining of Laminin 5 alpha 3 in HepG2 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1701-99, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
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Fig3:
Western blot analysis of Laminin 5 alpha 3 on different lysates with Rabbit anti-Laminin 5 alpha 3 antibody (ET1701-99) at 1/1,000 dilution. Lane 1: HeLa cell lysate Exposure time: 110 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ET1701-99, 1/1,000 in primary antibody dilution buffer (K1803)， overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 367 kDa Observed band size: 367kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.