Anti-Laminnin 5 alpha 3 antibody [JJ0957]
cat.: ET1701-99
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB, ICC/IF
Clonality: Monoclonal
Clone number: JJ0957
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Protein A affinity purified.
Molecular weight: 367 kDa
Isotype: IgG
Immunogen: Recombinant protein within human Laminnin 5 alpha 3 aa 50-150.
Positive control: A431 cell lysates, Hela, HepG2.
Subcellular location: Secreted.
Recommended Dilutions:
  WB
  ICC/IF

1:500-1:1,000
1:100-1:500
Uniprot #: SwissProt: Q16787 Human
Alternative names: E170 Epiligrin 170 kDa subunit Epiligrin subunit alpha Kalinin subunit alpha LAMA3 LAMA3A Laminin 5 alpha3 Laminin A3 laminin alpha 3 laminin alpha3 Laminin subunit alpha-3 Laminin-5 subunit alpha Laminin-6 subunit alpha Laminin-7 subunit alpha laminin5 alpha 3 Laminin5 alpha3 LAMNA LOCS Nicein subunit alpha
Images
ET1701-99_1.jpg Fig1: Western blot analysis of Laminnin 5 alpha 3 on A431 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1701-99, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
ET1701-99_2.jpg Fig2: ICC staining of Laminnin 5 alpha 3 in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1701-99, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1701-99_3.jpg Fig3: ICC staining of Laminnin 5 alpha 3 in HepG2 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1701-99, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.