Mitofusin 1 Recombinant Rabbit Monoclonal Antibody [JF0954]
cat.: ET1702-01
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IP |
| Clonality: | Monoclonal |
| Clone number: | JF0954 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 84 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human Mitofusin 1 aa 491-620 / 741. |
| Positive control: | K-562 cell lysates. |
| Subcellular location: | Mitochondrion outer membrane, Cytoplasm. |
| Recommended Dilutions:
WB |
1:1,000-1:2,000 |
| Uniprot #: | SwissProt: Q8IWA4 Human |
| Alternative names: | Fzo homolog Hfzo1 Hfzo2 MFN 1 Mfn1 MFN1_HUMAN Mitochondrial transmembrane GTPase Fzo 1 Mitochondrial transmembrane GTPase FZO 2 Mitochondrial transmembrane GTPase FZO1B Mitofusin 1 Mitofusin-1 Mitofusin1 MS996 Putative transmembrane GTPase Transmembrane GTPase MFN1 |
Images
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Fig1:
Western blot analysis of Mitofusin 1 on K-562 cell lysates with Rabbit anti-Mitofusin 1 antibody (ET1702-01) at 1/2,000 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 84 kDa Observed band size: 84 kDa Exposure time: 20 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1702-01) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.