Mitofusin 1 Recombinant Rabbit Monoclonal Antibody [JF0954]
cat.: ET1702-01
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB, IP, FC
Clonality: Monoclonal
Clone number: JF0954
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: 84 kDa
Isotype: IgG
Immunogen: Recombinant protein within Human Mitofusin 1 aa 491-620 / 741.
Positive control: K562 cell lysates, 293.
Subcellular location: Mitochondrion outer membrane, Cytoplasm.
Recommended Dilutions:
  WB
  FC

1:1,000-1:2,000
1:50-1:100
Uniprot #: SwissProt: Q8IWA4 Human
Alternative names: Fzo homolog Hfzo1 Hfzo2 MFN 1 Mfn1 MFN1_HUMAN Mitochondrial transmembrane GTPase Fzo 1 Mitochondrial transmembrane GTPase FZO 2 Mitochondrial transmembrane GTPase FZO1B Mitofusin 1 Mitofusin-1 Mitofusin1 MS996 Putative transmembrane GTPase Transmembrane GTPase MFN1
Images
ET1702-01_1.jpg Fig1: Western blot analysis of Mitofusin 1 on K562 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1702-01, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
ET1702-01_2.jpg Fig2: Flow cytometric analysis of Mitofusin 1 was done on 293 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1702-01, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.