Mitofusin 1 Recombinant Rabbit Monoclonal Antibody [JF0954]
cat.: ET1702-01
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IP, FC |
| Clonality: | Monoclonal |
| Clone number: | JF0954 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 84 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human Mitofusin 1 aa 491-620 / 741. |
| Positive control: | K562 cell lysates, 293. |
| Subcellular location: | Mitochondrion outer membrane, Cytoplasm. |
| Recommended Dilutions:
WB FC |
1:1,000-1:2,000 1:50-1:100 |
| Uniprot #: | SwissProt: Q8IWA4 Human |
| Alternative names: | Fzo homolog Hfzo1 Hfzo2 MFN 1 Mfn1 MFN1_HUMAN Mitochondrial transmembrane GTPase Fzo 1 Mitochondrial transmembrane GTPase FZO 2 Mitochondrial transmembrane GTPase FZO1B Mitofusin 1 Mitofusin-1 Mitofusin1 MS996 Putative transmembrane GTPase Transmembrane GTPase MFN1 |
Images
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Fig1: Western blot analysis of Mitofusin 1 on K562 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1702-01, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. |
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Fig2: Flow cytometric analysis of Mitofusin 1 was done on 293 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1702-01, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.