Transferrin Receptor (CD71) Recombinant Rabbit Monoclonal Antibody [JF0956]
cat.: ET1702-06
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IP, FC |
| Clonality: | Monoclonal |
| Clone number: | JF0956 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 85 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human CD71 aa 22-60 / 760. |
| Positive control: | HeLa cell lysate, K-562 cell lysate, SW480 cell lysate, U-87 MG cell lysate, RAW264.7 cell lysate, mouse spleen tissue lysate, rat spleen tissue lysate, Hela. |
| Subcellular location: | Cell membrane, Melanosome, Secreted. |
| Recommended Dilutions:
WB FC IP |
1:1,000-1:5,000 1:50-1:100 Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: P02786 Human | Q62351 Mouse | Q99376 Rat |
| Alternative names: | CD 71 CD71 CD71 antigen IMD46 OTTHUMP00000208523 OTTHUMP00000208524 OTTHUMP00000208525 p90 sTfR T9 TFR 1 TfR TfR1 TFR1_HUMAN TFRC TR Transferrin receptor (p90 CD71) Transferrin receptor protein 1, serum form Trfr |
Images
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Fig1:
Western blot analysis of Transferrin Receptor (CD71) on different lysates with Rabbit anti-Transferrin Receptor (CD71) antibody (ET1702-06) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: K-562 cell lysate Lane 3: SW480 cell lysate Lane 4: U-87 MG cell lysate Lane 5: RAW264.7 cell lysate Lane 6: Mouse spleen tissue lysate Lane 7: Rat spleen tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 85 kDa Observed band size: 100 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1702-06) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of Transferrin Receptor (CD71) on different lysates with Rabbit anti-Transferrin Receptor (CD71) antibody (ET1702-06) at 1/1,000 dilution. Lane 1: HeLa-si NT cell lysate Lane 2: HeLa-si Transferrin Receptor cell lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 85 kDa Observed band size: 100 kDa Exposure time: 1 minute 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1702-06) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig3:
Western blot analysis of Transferrin Receptor (CD71) on different lysates with Rabbit anti-Transferrin Receptor (CD71) antibody (ET1702-06) at 1/5,000 dilution. Lane 1: HAP1 WT cell lysate Lane 2: HAP1 Transferrin Receptor KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 85 kDa Observed band size: 90 kDa Exposure time: 1 minute 48 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1702-06) at 1/5,000 dilution was used in TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig4:
Flow cytometric analysis of HeLa cells labeling Transferrin Receptor (CD71). Cells were fixed and permeabilized. Then stained with the primary antibody (ET1702-06, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.