Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
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Species reactivity: | Human, Mouse, Rat |
Applications: | WB, IHC-P, IP, FC |
Clonality: | Monoclonal |
Clone number: | JF0956 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Protein A affinity purified. |
Molecular weight: | Predicted band size: 85 kDa |
Isotype: | IgG |
Immunogen: | Synthetic peptide within Human CD71 aa 22-60 / 760. |
Positive control: | HeLa cell lysate, K-562 cell lysate, SW480 cell lysate, U-87 MG cell lysate, RAW264.7 cell lysate, mouse spleen tissue lysate, rat spleen tissue lysate, rat brain tissue, human lung carcinoma tissue, mouse brain tissue, mouse placenta tissue, Hela. |
Subcellular location: | Cell membrane, Melanosome, Secreted. |
Recommended Dilutions:
WB IHC-P FC IP |
1:1,000-1:2,000 1:50-1:200 1:50-1:100 Use at an assay dependent concentration. |
Uniprot #: | SwissProt: P02786 Human | Q62351 Mouse | Q99376 Rat |
Alternative names: | CD 71 CD71 CD71 antigen IMD46 OTTHUMP00000208523 OTTHUMP00000208524 OTTHUMP00000208525 p90 sTfR T9 TFR 1 TfR TfR1 TFR1_HUMAN TFRC TR Transferrin receptor (p90 CD71) Transferrin receptor protein 1, serum form Trfr |
Fig1:
Western blot analysis of Transferrin Receptor (CD71) on different lysates with Rabbit anti-Transferrin Receptor (CD71) antibody (ET1702-06) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: K-562 cell lysate Lane 3: SW480 cell lysate Lane 4: U-87 MG cell lysate Lane 5: RAW264.7 cell lysate Lane 6: Mouse spleen tissue lysate Lane 7: Rat spleen tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 85 kDa Observed band size: 100 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1702-06) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of Transferrin Receptor (CD71) on different lysates with Rabbit anti-Transferrin Receptor (CD71) antibody (ET1702-06) at 1/1,000 dilution. Lane 1: HeLa-si NT cell lysate Lane 2: HeLa-si Transferrin Receptor cell lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 85 kDa Observed band size: 100 kDa Exposure time: 1 minute 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1702-06) at 1/1,000 dilution was used in antibody diluent at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Fig3: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Transferrin Receptor (CD71) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-06, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. | |
Fig4: Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using anti-Transferrin Receptor (CD71) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-06, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. | |
Fig5: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Transferrin Receptor (CD71) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-06, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. | |
Fig6: Immunohistochemical analysis of paraffin-embedded mouse placenta tissue using anti-Transferrin Receptor (CD71) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-06, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Fig7:
Flow cytometric analysis of HeLa cells labeling Transferrin Receptor (CD71). Cells were fixed and permeabilized. Then stained with the primary antibody (ET1702-06, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |