ABL2 Recombinant Rabbit Monoclonal Antibody [JF0951]
cat.: ET1702-07
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Rat, Mouse |
| Applications: | WB, FC |
| Clonality: | Monoclonal |
| Clone number: | JF0951 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 128 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human ABL2 aa 502-552 / 1182. |
| Positive control: | Hela cell lysate, PC-12 cell lysate, Jurkat cell lysate, Raji. |
| Subcellular location: | Cytoplasm, cytoskeleton. |
| Recommended Dilutions:
WB FC |
1:1,000-1:2,000 1:50-1:100 |
| Uniprot #: | SwissProt: P42684 Human | Q4JIM5 Mouse Unigene: 69847 Rat |
| Alternative names: | Abelson murine leukemia viral oncogene homolog 2 Abelson related gene protein Abelson tyrosine-protein kinase 2 Abelson-related gene protein ABL2 ABL2_HUMAN ABLL ARG Tyrosine kinase ARG Tyrosine protein kinase ABL2 Tyrosine-protein kinase ARG v abl Abelson murine leukemia viral oncogene homolog 2 |
Images
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Fig1:
Western blot analysis of ABL2 on different lysates with Rabbit anti-ABL2 antibody (ET1702-07) at 1/2,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-ABL2 KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 128 kDa Observed band size: 128 kDa Exposure time: 30 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1702-07) at 1/2,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of ABL2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1702-07, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Hela cell lysate Lane 2: PC-12 cell lysate Lane 3: Jurkat cell lysate Predicted band size: 128 kDa Observed band size: 128 kDa |
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Fig3: Flow cytometric analysis of ABL2 was done on Raji cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1702-07, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.