Peroxiredoxin 1 Recombinant Rabbit Monoclonal Antibody [JF0945]
cat.: ET1702-08
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IF-Tissue, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JF0945 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 22 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within N-terminal human Peroxiredoxin 1. |
| Positive control: | HeLa cell lysate, NIH/3T3 cell lysate, Mouse brain tissue lysate, human liver cancer tissue, mouse kidney tissue, human kidney tissue. |
| Subcellular location: | Cytoplasm, Melanosome. |
| Recommended Dilutions:
WB IF-Tissue IHC-P |
1:1,000-1:5,000 1:100-1:500 1:200-1:1,000 |
| Uniprot #: | SwissProt: Q06830 Human | P35700 Mouse |
| Alternative names: | Heme binding 23 kDa protein MSP23 Natural killer cell-enhancing factor A NKEF A NKEF-A NKEFA OSF3 Osteoblast specific factor 3 PAG Paga PAGB Peroxiredoxin-1 PRDX1 PRDX1_HUMAN Proliferation associated gene A Proliferation-associated gene protein PRX1 PrxI TDPX2 Thioredoxin peroxidase 2 Thioredoxin-dependent peroxide reductase 2 |
Images
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Fig1:
Western blot analysis of Peroxiredoxin 1 on different lysates with Rabbit anti-Peroxiredoxin 1 antibody (ET1702-08) at 1/1,000 dilution. Lane 1: HeLa cell lysate (10 µg/Lane) Lane 2: NIH/3T3 cell lysate (10 µg/Lane) Lane 3: Mouse brain tissue lysate (20 µg/Lane) Predicted band size: 22 kDa Observed band size: 22 kDa Exposure time: 46 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1702-08) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue with Rabbit anti-Peroxiredoxin 1 antibody (ET1702-08) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-08) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-Peroxiredoxin 1 antibody (ET1702-08) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-08) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Peroxiredoxin 1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-08, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.