Glutathione Peroxidase 1 Recombinant Rabbit Monoclonal Antibody [JF0944]
cat.: ET1702-09
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IF-Tissue, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JF0944 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 22 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Glutathione Peroxidase 1 aa 1-50 / 203. |
| Positive control: | SH-SY5Y cell lysate, THP-1 cell lysate, HEK-293 cell lysate, human liver tissue, human kidney tissue, mouse colon tissue, human tonsil tissue, human breast carcinoma tissue. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB IF-Tissue IHC-P |
1:1,000-1:2,000 1:50-1:200 1:50-1:1,000 |
| Uniprot #: | SwissProt: P07203 Human | P11352 Mouse |
| Alternative names: | AL033363 Cellular glutathione peroxidase Glutathione peroxidase 1 Glutathione peroxidase GPx 1 GPx-1 GPX1 GPX1_HUMAN GPXD GSHPx-1 GSHPX1 MGC14399 MGC88245 |
Images
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Fig1:
Western blot analysis of Glutathione Peroxidase 1 on different lysates with Rabbit anti-Glutathione Peroxidase 1 antibody (ET1702-09) at 1/1,000 dilution. Lane 1: SH-SY5Y cell lysate Lane 2: THP-1 cell lysate Lane 3: HEK-293 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 22 kDa Observed band size: 22 kDa Exposure time: 25 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1702-09) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Glutathione Peroxidase 1 antibody (ET1702-09) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-09) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Glutathione Peroxidase 1 antibody. Counter stained with hematoxylin. |
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Fig4: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Glutathione Peroxidase 1 antibody. Counter stained with hematoxylin. |
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Fig5: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Glutathione Peroxidase 1 antibody. Counter stained with hematoxylin. |
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Fig6: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Glutathione Peroxidase 1 antibody. Counter stained with hematoxylin. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.