Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
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Species reactivity: | Human, Mouse, Rat, Zebrafish |
Applications: | WB, FC |
Clonality: | Monoclonal |
Clone number: | JF0952 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Protein A affinity purified. |
Molecular weight: | 86 kDa |
Isotype: | IgG |
Immunogen: | Recombinant protein within Human Glucocorticoid Receptor aa 2-220 / 777. |
Positive control: | Zebrafish tissue lysate, A549 cell lysate, HepG2 cell lysate, hybrid fish (crucian-carp) heart tissue lysates, NIH/3T3. |
Subcellular location: | Cytoplasm, Nucleus, Mitochondrion. |
Recommended Dilutions:
WB FC |
1:1,000-1:5,000 1:50-1:100 |
Uniprot #: | SwissProt: P04150 Human | P06537 Mouse | P06536 Rat |
Alternative names: | GCCR GCR GCR_HUMAN GCRST glucocorticoid nuclear receptor variant 1 Glucocorticoid receptor GR GRL Grl1 nr3c1 Nuclear receptor subfamily 3 group C member 1 nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor) |
Fig1:
Western blot analysis of Glucocorticoid Receptor on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1702-11, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:40,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Zebrafish tissue lysate Lane 2: A549 cell lysate Lane 3: HepG2 cell lysate |
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Fig2: Western blot analysis of Glucocorticoid Receptor on hybrid fish (crucian-carp) heart tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1702-11, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:40,000 dilution was used for 1 hour at room temperature. | |
Fig3: Flow cytometric analysis of Glucocorticoid Receptor was done on NIH/3T3 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1702-11, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |