Glucocorticoid Receptor Recombinant Rabbit Monoclonal Antibody [JF0952]
cat.: ET1702-11
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat, Zebrafish
Applications: WB, FC
Clonality: Monoclonal
Clone number: JF0952
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Protein A affinity purified.
Molecular weight: 86 kDa
Isotype: IgG
Immunogen: Recombinant protein within Human Glucocorticoid Receptor aa 2-220 / 777.
Positive control: Zebrafish tissue lysate, A549 cell lysate, HepG2 cell lysate, hybrid fish (crucian-carp) heart tissue lysates, NIH/3T3.
Subcellular location: Cytoplasm, Nucleus, Mitochondrion.
Recommended Dilutions:
  WB
  FC

1:1,000-1:5,000
1:50-1:100
Uniprot #: SwissProt: P04150 Human | P06537 Mouse | P06536 Rat
Alternative names: GCCR GCR GCR_HUMAN GCRST glucocorticoid nuclear receptor variant 1 Glucocorticoid receptor GR GRL Grl1 nr3c1 Nuclear receptor subfamily 3 group C member 1 nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor)
Images
ET1702-11_1.jpg Fig1: Western blot analysis of Glucocorticoid Receptor on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1702-11, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:40,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Zebrafish tissue lysate
Lane 2: A549 cell lysate
Lane 3: HepG2 cell lysate
ET1702-11_2.jpg Fig2: Western blot analysis of Glucocorticoid Receptor on hybrid fish (crucian-carp) heart tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1702-11, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:40,000 dilution was used for 1 hour at room temperature.
ET1702-11_3.jpg Fig3: Flow cytometric analysis of Glucocorticoid Receptor was done on NIH/3T3 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1702-11, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.