Anti-FOXP3 antibody [JF0898]
cat.: ET1702-12
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB, ICC, FC
Clonality: Monoclonal
Clone number: JF0898
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Protein A affinity purified.
Molecular weight: 47 kDa
Isotype: IgG
Immunogen: Recombinant protein within human FOXP3 aa 250-431.
Positive control: K562 cell lysates, MCF-7, 293T, Jurkat.
Subcellular location: Nucleus, Cytoplasm.
Recommended Dilutions:
  WB
  ICC
  FC

1:500-1:2,000
1:50-1:100
1:50-1:100
Uniprot #: SwissProt: Q9BZS1 Human
Alternative names: AIID antibody DIETER antibody Forkhead box P3 antibody Forkhead box protein P3 antibody FOXP3 antibody FOXP3_HUMAN antibody FOXP3delta7 antibody Immune dysregulation polyendocrinopathy enteropathy X linked antibody Immunodeficiency polyendocrinopathy enteropathy X linked antibody IPEX antibody JM2 antibody MGC141961 antibody MGC141963 antibody OTTHUMP00000025832 antibody OTTHUMP00000025833 antibody OTTHUMP00000226737 antibody PIDX antibody Scurfin antibody XPID antibody
Images
ET1702-12_1.jpg Fig1: Western blot analysis of FOXP3 on K562 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1702-12, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
ET1702-12_2.jpg Fig2: ICC staining of FOXP3 in MCF-7 cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1702-12, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®594 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1702-12_3.jpg Fig3: ICC staining of FOXP3 in 293T cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1702-12, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®594 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1702-12_4.jpg Fig4: Flow cytometric analysis of FOXP3 was done on Jurkat cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1702-12, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.